Functional analysis of genetic variants in the high-risk breast cancer susceptibility gene PALB2

Rick A.C.M. Boonen; Amélie Rodrigue; Chantal Stoepker; Wouter W. Wiegant; Bas Vroling; Milan Sharma; Magdalena B. Rother; Nandi Celosse; Maaike P.G. Vreeswijk; Fergus Couch; Jacques Simard; Peter Devilee; Jean Yves Masson; Haico van Attikum

doi:10.1038/s41467-019-13194-2

Functional analysis of genetic variants in the high-risk breast cancer susceptibility gene PALB2

Rick A.C.M. Boonen, Amélie Rodrigue, Chantal Stoepker, Wouter W. Wiegant, Bas Vroling, Milan Sharma, Magdalena B. Rother, Nandi Celosse, Maaike P.G. Vreeswijk, Fergus Couch, Jacques Simard, Peter Devilee, Jean Yves Masson, Haico van Attikum

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15 Scopus citations

Abstract

Heterozygous carriers of germ-line loss-of-function variants in the DNA repair gene PALB2 are at a highly increased lifetime risk for developing breast cancer. While truncating variants in PALB2 are known to increase cancer risk, the interpretation of missense variants of uncertain significance (VUS) is in its infancy. Here we describe the development of a relatively fast and easy cDNA-based system for the semi high-throughput functional analysis of 48 VUS in human PALB2. By assessing the ability of PALB2 VUS to rescue the DNA repair and checkpoint defects in Palb2 knockout mouse embryonic stem (mES) cells, we identify various VUS in PALB2 that impair its function. Three VUS in the coiled-coil domain of PALB2 abrogate the interaction with BRCA1, whereas several VUS in the WD40 domain dramatically reduce protein stability. Thus, our functional assays identify damaging VUS in PALB2 that may increase cancer risk.

Original language	English (US)
Article number	5296
Journal	Nature communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-13194-2
State	Published - Dec 1 2019

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General
General Physics and Astronomy

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Boonen, R. A. C. M., Rodrigue, A., Stoepker, C., Wiegant, W. W., Vroling, B., Sharma, M., Rother, M. B., Celosse, N., Vreeswijk, M. P. G., Couch, F., Simard, J., Devilee, P., Masson, J. Y., & van Attikum, H. (2019). Functional analysis of genetic variants in the high-risk breast cancer susceptibility gene PALB2. Nature communications, 10(1), Article 5296. https://doi.org/10.1038/s41467-019-13194-2

Boonen, RACM, Rodrigue, A, Stoepker, C, Wiegant, WW, Vroling, B, Sharma, M, Rother, MB, Celosse, N, Vreeswijk, MPG, Couch, F, Simard, J, Devilee, P, Masson, JY & van Attikum, H 2019, 'Functional analysis of genetic variants in the high-risk breast cancer susceptibility gene PALB2', Nature communications, vol. 10, no. 1, 5296. https://doi.org/10.1038/s41467-019-13194-2

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abstract = "Heterozygous carriers of germ-line loss-of-function variants in the DNA repair gene PALB2 are at a highly increased lifetime risk for developing breast cancer. While truncating variants in PALB2 are known to increase cancer risk, the interpretation of missense variants of uncertain significance (VUS) is in its infancy. Here we describe the development of a relatively fast and easy cDNA-based system for the semi high-throughput functional analysis of 48 VUS in human PALB2. By assessing the ability of PALB2 VUS to rescue the DNA repair and checkpoint defects in Palb2 knockout mouse embryonic stem (mES) cells, we identify various VUS in PALB2 that impair its function. Three VUS in the coiled-coil domain of PALB2 abrogate the interaction with BRCA1, whereas several VUS in the WD40 domain dramatically reduce protein stability. Thus, our functional assays identify damaging VUS in PALB2 that may increase cancer risk.",

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Functional analysis of genetic variants in the high-risk breast cancer susceptibility gene PALB2

Abstract

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