Fecal bile acids, short-chain fatty acids, and bacteria after ileal pouch-anal anastomosis do not differ in patients with pouchitis

William J. Sandborn, William J. Tremaine, Kenneth P. Batts, John H. Pemberton, Steven S. Rossi, Alan F. Hofmann, Gregory James Gores, Sidney F. Phillips

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Construction of an ileal reservoir changes the fecal bacterial flora and the fecal composition of bile acids and short-chain fatty acids. We examined the relationships between pouch inflammation (pouchitis) and pouch content, as assessed by analysis of fecal bacteria, bile acids, and short chain fatty acids. Four groups were studied: ileal pouch-anal anastomosis (IPAA) for ulcerative colitis with pouchitis (N=10), IPAA without pouchitis (N=5), IPAA for familial adenomatous polyposis without pouchitis (N=5); and Brooke ileostomy for ulcerative colitis, which served as controls (N=5). Pouchitis was defined as ≥7 points on an 18-point pouchitis disease activity index. Aerobic and anaerobic bacteria were quantitatively cultured. Total aqueous-phase bile acids were measured by thin-layer chromatography and an enzymatic 3α-OH hydroxysteroid dehydrogenase method. Fecal short chain fatty acids were measured by gas liquid chromatography. All patients with an IPAA had higher ratios of anaerobes/aerobes and concentrations of anaerobic gram-negative rods than did patients with an ileostomy. There were no other differences between patient groups with respect to bacteria, aqueous-phase total bile acids, or fecal short-chain fatty acids. Fecal concentrations of bacteria, bile acids, and short-chain fatty acids were similar in patients with and without pouchitis, indicating that these factors can not be the sole cause of pouchitis.

Original languageEnglish (US)
Pages (from-to)1474-1483
Number of pages10
JournalDigestive Diseases and Sciences
Volume40
Issue number7
DOIs
StatePublished - Jul 1995

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Colonic Pouches
Volatile Fatty Acids
Bile Acids and Salts
Inflammation
Bacteria
Ileostomy
Ulcerative Colitis
3-Hydroxysteroid Dehydrogenases
Pouchitis
Aerobic Bacteria
Adenomatous Polyposis Coli
Anaerobic Bacteria
Thin Layer Chromatography
Gas Chromatography

Keywords

  • bile acids
  • familial adenomatous polyposis
  • ileal pouch-anal anastomosis
  • pouchitis
  • shortchain fatty acids
  • ulcerative colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Sandborn, W. J., Tremaine, W. J., Batts, K. P., Pemberton, J. H., Rossi, S. S., Hofmann, A. F., ... Phillips, S. F. (1995). Fecal bile acids, short-chain fatty acids, and bacteria after ileal pouch-anal anastomosis do not differ in patients with pouchitis. Digestive Diseases and Sciences, 40(7), 1474-1483. https://doi.org/10.1007/BF02285195

Fecal bile acids, short-chain fatty acids, and bacteria after ileal pouch-anal anastomosis do not differ in patients with pouchitis. / Sandborn, William J.; Tremaine, William J.; Batts, Kenneth P.; Pemberton, John H.; Rossi, Steven S.; Hofmann, Alan F.; Gores, Gregory James; Phillips, Sidney F.

In: Digestive Diseases and Sciences, Vol. 40, No. 7, 07.1995, p. 1474-1483.

Research output: Contribution to journalArticle

Sandborn, William J. ; Tremaine, William J. ; Batts, Kenneth P. ; Pemberton, John H. ; Rossi, Steven S. ; Hofmann, Alan F. ; Gores, Gregory James ; Phillips, Sidney F. / Fecal bile acids, short-chain fatty acids, and bacteria after ileal pouch-anal anastomosis do not differ in patients with pouchitis. In: Digestive Diseases and Sciences. 1995 ; Vol. 40, No. 7. pp. 1474-1483.
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