Facile assembly of micro- and nanoarrays for sensing with natural cell membranes

Nathan J. Wittenberg; Hyungsoon Im; Timothy W. Johnson; Xiaohua Xu; Arthur E. Warrington; Moses Rodriguez; Sang Hyun Oh

doi:10.1021/nn202554t

Facile assembly of micro- and nanoarrays for sensing with natural cell membranes

Nathan J. Wittenberg, Hyungsoon Im, Timothy W. Johnson, Xiaohua Xu, Arthur E. Warrington, Moses Rodriguez, Sang Hyun Oh

Neurology

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Microarray technology has facilitated many powerful high-throughput studies in the fields of genetics and proteomics, among others. However, preparation of microarrays composed of cell-derived membranes with embedded receptors has proven difficult. Here we describe a new method for forming microarrays composed of synthetic lipid vesicles and natural cell membranes. The method is based upon assembly of vesicles and natural membranes into recessed micro- and nanowells and using a polydimethylsiloxane (PDMS) block as a "squeegee." This method is used to assemble phospholipid vesicles into arrays with micrometer and nanoscale dimensions. Native myelin and neuronal lipid raft arrays are also formed in 30 min or less. We show the natural membrane arrays can be used for sensing lipid-protein interactions by detecting cholera toxin binding to ganglioside GM1 in neuronal lipid rafts. In multicomponent arrays myelin can be distinguished from neuronal rafts by antibody binding to cell-specific surface antigens. Finally, myelin arrays formed in gold nanowells are used for surface plasmon resonance sensing. This assembly approach is simple, broadly applicable, and opens up new avenues of research not easily accomplished with standard microarray technology.

Original language	English (US)
Pages (from-to)	7555-7564
Number of pages	10
Journal	ACS Nano
Volume	5
Issue number	9
DOIs	https://doi.org/10.1021/nn202554t
State	Published - Sep 27 2011

Keywords

atomic layer deposition
lipid rafts
microarray
myelin
nanoimprint lithography
surface plasmon resonance
vesicle array

ASJC Scopus subject areas

General Materials Science
General Engineering
General Physics and Astronomy

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10.1021/nn202554t

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abstract = "Microarray technology has facilitated many powerful high-throughput studies in the fields of genetics and proteomics, among others. However, preparation of microarrays composed of cell-derived membranes with embedded receptors has proven difficult. Here we describe a new method for forming microarrays composed of synthetic lipid vesicles and natural cell membranes. The method is based upon assembly of vesicles and natural membranes into recessed micro- and nanowells and using a polydimethylsiloxane (PDMS) block as a {"}squeegee.{"} This method is used to assemble phospholipid vesicles into arrays with micrometer and nanoscale dimensions. Native myelin and neuronal lipid raft arrays are also formed in 30 min or less. We show the natural membrane arrays can be used for sensing lipid-protein interactions by detecting cholera toxin binding to ganglioside GM1 in neuronal lipid rafts. In multicomponent arrays myelin can be distinguished from neuronal rafts by antibody binding to cell-specific surface antigens. Finally, myelin arrays formed in gold nanowells are used for surface plasmon resonance sensing. This assembly approach is simple, broadly applicable, and opens up new avenues of research not easily accomplished with standard microarray technology.",

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AU - Wittenberg, Nathan J.

AU - Im, Hyungsoon

AU - Johnson, Timothy W.

AU - Xu, Xiaohua

AU - Warrington, Arthur E.

AU - Rodriguez, Moses

AU - Oh, Sang Hyun

PY - 2011/9/27

Y1 - 2011/9/27

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AB - Microarray technology has facilitated many powerful high-throughput studies in the fields of genetics and proteomics, among others. However, preparation of microarrays composed of cell-derived membranes with embedded receptors has proven difficult. Here we describe a new method for forming microarrays composed of synthetic lipid vesicles and natural cell membranes. The method is based upon assembly of vesicles and natural membranes into recessed micro- and nanowells and using a polydimethylsiloxane (PDMS) block as a "squeegee." This method is used to assemble phospholipid vesicles into arrays with micrometer and nanoscale dimensions. Native myelin and neuronal lipid raft arrays are also formed in 30 min or less. We show the natural membrane arrays can be used for sensing lipid-protein interactions by detecting cholera toxin binding to ganglioside GM1 in neuronal lipid rafts. In multicomponent arrays myelin can be distinguished from neuronal rafts by antibody binding to cell-specific surface antigens. Finally, myelin arrays formed in gold nanowells are used for surface plasmon resonance sensing. This assembly approach is simple, broadly applicable, and opens up new avenues of research not easily accomplished with standard microarray technology.

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KW - vesicle array

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Facile assembly of micro- and nanoarrays for sensing with natural cell membranes

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Keywords

ASJC Scopus subject areas

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