Facile assembly of micro- and nanoarrays for sensing with natural cell membranes

Nathan J. Wittenberg, Hyungsoon Im, Timothy W. Johnson, Xiaohua Xu, Arthur E. Warrington, Moses Rodriguez, Sang Hyun Oh

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Microarray technology has facilitated many powerful high-throughput studies in the fields of genetics and proteomics, among others. However, preparation of microarrays composed of cell-derived membranes with embedded receptors has proven difficult. Here we describe a new method for forming microarrays composed of synthetic lipid vesicles and natural cell membranes. The method is based upon assembly of vesicles and natural membranes into recessed micro- and nanowells and using a polydimethylsiloxane (PDMS) block as a "squeegee." This method is used to assemble phospholipid vesicles into arrays with micrometer and nanoscale dimensions. Native myelin and neuronal lipid raft arrays are also formed in 30 min or less. We show the natural membrane arrays can be used for sensing lipid-protein interactions by detecting cholera toxin binding to ganglioside GM1 in neuronal lipid rafts. In multicomponent arrays myelin can be distinguished from neuronal rafts by antibody binding to cell-specific surface antigens. Finally, myelin arrays formed in gold nanowells are used for surface plasmon resonance sensing. This assembly approach is simple, broadly applicable, and opens up new avenues of research not easily accomplished with standard microarray technology.

Original languageEnglish (US)
Pages (from-to)7555-7564
Number of pages10
JournalACS Nano
Volume5
Issue number9
DOIs
StatePublished - Sep 27 2011

Fingerprint

Cell membranes
Microarrays
Lipids
myelin
rafts
assembly
lipids
Membranes
membranes
G(M1) Ganglioside
cholera
Cholera Toxin
Phospholipids
Surface plasmon resonance
Polydimethylsiloxane
Surface Antigens
Antigens
Antibodies
Gold
antigens

Keywords

  • atomic layer deposition
  • lipid rafts
  • microarray
  • myelin
  • nanoimprint lithography
  • surface plasmon resonance
  • vesicle array

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Wittenberg, N. J., Im, H., Johnson, T. W., Xu, X., Warrington, A. E., Rodriguez, M., & Oh, S. H. (2011). Facile assembly of micro- and nanoarrays for sensing with natural cell membranes. ACS Nano, 5(9), 7555-7564. https://doi.org/10.1021/nn202554t

Facile assembly of micro- and nanoarrays for sensing with natural cell membranes. / Wittenberg, Nathan J.; Im, Hyungsoon; Johnson, Timothy W.; Xu, Xiaohua; Warrington, Arthur E.; Rodriguez, Moses; Oh, Sang Hyun.

In: ACS Nano, Vol. 5, No. 9, 27.09.2011, p. 7555-7564.

Research output: Contribution to journalArticle

Wittenberg, NJ, Im, H, Johnson, TW, Xu, X, Warrington, AE, Rodriguez, M & Oh, SH 2011, 'Facile assembly of micro- and nanoarrays for sensing with natural cell membranes', ACS Nano, vol. 5, no. 9, pp. 7555-7564. https://doi.org/10.1021/nn202554t
Wittenberg NJ, Im H, Johnson TW, Xu X, Warrington AE, Rodriguez M et al. Facile assembly of micro- and nanoarrays for sensing with natural cell membranes. ACS Nano. 2011 Sep 27;5(9):7555-7564. https://doi.org/10.1021/nn202554t
Wittenberg, Nathan J. ; Im, Hyungsoon ; Johnson, Timothy W. ; Xu, Xiaohua ; Warrington, Arthur E. ; Rodriguez, Moses ; Oh, Sang Hyun. / Facile assembly of micro- and nanoarrays for sensing with natural cell membranes. In: ACS Nano. 2011 ; Vol. 5, No. 9. pp. 7555-7564.
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