TY - JOUR
T1 - Fabrication and characterization of an inorganic gold and silica nanoparticle mediated drug delivery system for nitric oxide.
AU - Das, Amitava
AU - Mukherjee, Priyabrata
AU - Singla, Sumit K.
AU - Guturu, Praveen
AU - Frost, Megan C.
AU - Mukhopadhyay, Debabrata
AU - Shah, Vijay H.
AU - Patra, Chitta Ranjan
PY - 2010/7/30
Y1 - 2010/7/30
N2 - Nitric oxide (NO) plays an important role in inhibiting the development of hepatic fibrosis and its ensuing complication of portal hypertension by inhibiting human hepatic stellate cell (HSC) activation. Here we have developed a gold nanoparticle and silica nanoparticle mediated drug delivery system containing NO donors, which could be used for potential therapeutic application in chronic liver disease. The gold nanoconjugates were characterized using several physico-chemical techniques such as UV-visible spectroscopy and transmission electron microscopy. Silica nanoconjugates were synthesized and characterized as reported previously. NO released from gold and silica nanoconjugates was quantified under physiological conditions (pH = 7.4 at 37 degrees C) for a substantial period of time. HSC proliferation and the vascular tube formation ability, manifestations of their activation, were significantly attenuated by the NO released from these nanoconjugates. This study indicates that gold and silica nanoparticle mediated drug delivery systems for introducing NO could be used as a strategy for the treatment of hepatic fibrosis or chronic liver diseases, by limiting HSC activation.
AB - Nitric oxide (NO) plays an important role in inhibiting the development of hepatic fibrosis and its ensuing complication of portal hypertension by inhibiting human hepatic stellate cell (HSC) activation. Here we have developed a gold nanoparticle and silica nanoparticle mediated drug delivery system containing NO donors, which could be used for potential therapeutic application in chronic liver disease. The gold nanoconjugates were characterized using several physico-chemical techniques such as UV-visible spectroscopy and transmission electron microscopy. Silica nanoconjugates were synthesized and characterized as reported previously. NO released from gold and silica nanoconjugates was quantified under physiological conditions (pH = 7.4 at 37 degrees C) for a substantial period of time. HSC proliferation and the vascular tube formation ability, manifestations of their activation, were significantly attenuated by the NO released from these nanoconjugates. This study indicates that gold and silica nanoparticle mediated drug delivery systems for introducing NO could be used as a strategy for the treatment of hepatic fibrosis or chronic liver diseases, by limiting HSC activation.
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U2 - 10.1088/0957-4484/21/30/305102
DO - 10.1088/0957-4484/21/30/305102
M3 - Article
C2 - 20610873
AN - SCOPUS:79952200328
SN - 0957-4484
VL - 21
SP - 305102
JO - Nanotechnology
JF - Nanotechnology
IS - 30
ER -