External labeling of galactose in surface membrane glycoproteins of the intact myelin sheath

J. F. Poduslo, R. H. Quarles, R. O. Brady

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The molecular organization of surface galactose residues in glycoproteins of the intact myelin sheath was investigated using the enzymatic membrane probe, galactose oxidase. Rat spinal cords treated under physiological conditions with this nonpermeant probe were labeled specifically in galactose residues by reduction with tritiated sodium borohydride. The enzymatically modified proteins from isolated myelin were analyzed electrophoretically and their specific radioactivities determined. Results indicated tritium label associated with a surprising variety of high molecular weight proteins. The most extensively labeled peak corresponded to the major myelin glycoprotein as indicated by the coincidence of tritium label with that of [14C]fucose used as an internal marker for the glycoproteins. The radioactivity associated with this protein was 1.1 to 2.7 times higher after treatment with galactose oxidase when compared to reduction in the absence of the enzyme and 1.4 to 4.8 times higher when oxidized and reduced after prior treatment with neuraminidase. The results suggest a complex heterogeneity of minor glycoproteins associated with isolated myelin. It is concluded that from this complexity of glycoproteins, a major glycoprotein is at least partially localized on the external surface of either the intact myelin sheath or the closely associated oligodendroglial plasma membrane. Such a localization of this glycoprotein and the probable localization of the other glycoproteins enhances their potential role in specific interactions in the process of myelination or myelin maintenance.

Original languageEnglish (US)
Pages (from-to)153-158
Number of pages6
JournalJournal of Biological Chemistry
Volume251
Issue number1
StatePublished - 1976
Externally publishedYes

Fingerprint

Membrane Glycoproteins
Myelin Sheath
Galactose
Labeling
Glycoproteins
Galactose Oxidase
Tritium
Radioactivity
Labels
Myelin Proteins
Proteins
Fucose
Neuraminidase
Cell membranes
Rats
Spinal Cord
Molecular Weight
Molecular weight
Maintenance
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

External labeling of galactose in surface membrane glycoproteins of the intact myelin sheath. / Poduslo, J. F.; Quarles, R. H.; Brady, R. O.

In: Journal of Biological Chemistry, Vol. 251, No. 1, 1976, p. 153-158.

Research output: Contribution to journalArticle

Poduslo, J. F. ; Quarles, R. H. ; Brady, R. O. / External labeling of galactose in surface membrane glycoproteins of the intact myelin sheath. In: Journal of Biological Chemistry. 1976 ; Vol. 251, No. 1. pp. 153-158.
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