Expression profile of the matricellular protein osteopontin in primary open-angle glaucoma and the normal human eye

Uttio Roy Chowdhury, Seung Youn Jea, Dong Jin Oh, Douglas J. Rhee, Michael P Fautsch

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose. To characterize the role of osteopontin (OPN) in primary open-angle glaucoma (POAG) and normal eyes. Methods. OPN quantification was performed by enzyme-linked immunosorbent assay in aqueous humor (AH) obtained from human donor eyes (POAG and normal) and surgical samples (POAG and elective cataract removal). OPN expression and localization in whole eye tissue sections and primary normal human trabecular meshwork (NTM) cells were studied by Western blot and immunohistochemistry. Latanoprost-free acid (LFA)-treated NTM cells were analyzed for OPN gene and protein expression. Intraocular pressure was measured by tonometry, and central corneal thickness was measured by optical coherence tomography in young OPN-/- and wild-type mice. Results. OPN levels were significantly reduced in donor POAG AH compared with normal AH (0.54 ± 0.18 ng/μg [n = 8] vs. 0.77 ± 0.23 ng/μg [n = 9]; P = 0.039). A similar trend was observed in surgical AH (1.05 ± 0.31 ng/μg [n = 20] vs. 1.43 ± 0.88 ng/μg [n = 20]; P = 0.083). OPN was present in the trabecular meshwork, corneal epithelium and endothelium, iris, ciliary body, retina, vitreous humor, and optic nerve. LFA increased OPN gene expression, but minimal change in OPN protein expression was observed. No difference in intraocular pressure (17.5 ± 2.0 mm Hg [n = 56] vs. 17.3 ± 1.9 mm Hg [n = 68]) but thinner central corneal thickness (91.7 ± 3.6 μm [n = 50] vs. 99.2 ± 5.5 μm [n = 70]) was noted between OPN-/- and wild-type mice. Conclusions. OPN is widely distributed in the human eye and was found in lower concentrations in POAG AH. Reduction of OPN in young mice does not affect IOP. 2011 The Association for Research in Vision and Ophthalmology, Inc.

Original languageEnglish (US)
Pages (from-to)6443-6451
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number9
DOIs
StatePublished - Aug 2011

Fingerprint

Osteopontin
Aqueous Humor
Proteins
latanoprost
Trabecular Meshwork
Intraocular Pressure
Primary Open Angle Glaucoma
Gene Expression
Corneal Endothelium
Vitreous Body
Corneal Epithelium
Ciliary Body
Acids
Manometry
Optical Coherence Tomography
Iris
Optic Nerve
Cataract
Retina
Western Blotting

ASJC Scopus subject areas

  • Medicine(all)
  • Ophthalmology
  • Cellular and Molecular Neuroscience
  • Sensory Systems

Cite this

Expression profile of the matricellular protein osteopontin in primary open-angle glaucoma and the normal human eye. / Chowdhury, Uttio Roy; Jea, Seung Youn; Oh, Dong Jin; Rhee, Douglas J.; Fautsch, Michael P.

In: Investigative Ophthalmology and Visual Science, Vol. 52, No. 9, 08.2011, p. 6443-6451.

Research output: Contribution to journalArticle

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abstract = "Purpose. To characterize the role of osteopontin (OPN) in primary open-angle glaucoma (POAG) and normal eyes. Methods. OPN quantification was performed by enzyme-linked immunosorbent assay in aqueous humor (AH) obtained from human donor eyes (POAG and normal) and surgical samples (POAG and elective cataract removal). OPN expression and localization in whole eye tissue sections and primary normal human trabecular meshwork (NTM) cells were studied by Western blot and immunohistochemistry. Latanoprost-free acid (LFA)-treated NTM cells were analyzed for OPN gene and protein expression. Intraocular pressure was measured by tonometry, and central corneal thickness was measured by optical coherence tomography in young OPN-/- and wild-type mice. Results. OPN levels were significantly reduced in donor POAG AH compared with normal AH (0.54 ± 0.18 ng/μg [n = 8] vs. 0.77 ± 0.23 ng/μg [n = 9]; P = 0.039). A similar trend was observed in surgical AH (1.05 ± 0.31 ng/μg [n = 20] vs. 1.43 ± 0.88 ng/μg [n = 20]; P = 0.083). OPN was present in the trabecular meshwork, corneal epithelium and endothelium, iris, ciliary body, retina, vitreous humor, and optic nerve. LFA increased OPN gene expression, but minimal change in OPN protein expression was observed. No difference in intraocular pressure (17.5 ± 2.0 mm Hg [n = 56] vs. 17.3 ± 1.9 mm Hg [n = 68]) but thinner central corneal thickness (91.7 ± 3.6 μm [n = 50] vs. 99.2 ± 5.5 μm [n = 70]) was noted between OPN-/- and wild-type mice. Conclusions. OPN is widely distributed in the human eye and was found in lower concentrations in POAG AH. Reduction of OPN in young mice does not affect IOP. 2011 The Association for Research in Vision and Ophthalmology, Inc.",
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AU - Fautsch, Michael P

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