Expression of SH2D2A in T-cells is regulated both at the transcriptional and translational level

Kristin Melkevik Kolltveit, Stine Granum, Hans Christian Aasheim, Monika Forsbring, Vibeke Sundvold-Gjerstad, Ke Zheng Dai, Øyvind Molberg, Karoline W. Schjetne, Bjarne Bogen, Virginia S. Shapiro, Finn Eirik Johansen, Karl Schenck, Anne Spurkland

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The T-cell specific adapter protein (TSAd) encoded by the SH2D2A gene is up-regulated in activated human CD4+ T-cells in a cAMP-dependent manner. Expression of SH2D2A is important for proper activation of T-cells. Here, we show that SH2D2A expression is regulated both at the transcriptional and translational level. cAMP signaling alone induces TSAd-mRNA expression but fails to induce increased TSAd protein levels. By contrast, TCR engagement provides signals for both TSAd transcription and translation. We further show that cAMP signaling can prime T-cells for a more prompt expression of TSAd protein upon TCR stimulation. Our study thus points to a novel mechanism for how cAMP signaling may modulate T-cell activation through transcriptional priming of resting cells.

Original languageEnglish (US)
Pages (from-to)2380-2390
Number of pages11
JournalMolecular Immunology
Volume45
Issue number8
DOIs
StatePublished - Apr 1 2008

Keywords

  • Adapter protein
  • Gene expression regulation
  • T-cell activation
  • cAMP

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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