Expression of centrin isoforms in the mammalian retina

Uwe Wolfram, Jeffrey L Salisbury

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Centrin is a calcium-binding phosphoprotein of centrosomes, mitotic spindle poles, and flagellar basal apparatus. Indirect immunofluorescence studies in human and rat retinas reveal centrin localization in two distinct cellular structures: at centrosomes of nonciliated neuronal cells as well as in basal bodies, and in larger amounts in the highly modified cilium-the connecting cilium-of photoreceptor cells. Western blot analyses of mammalian retinal proteins show two closely migrating centrin bands at about 20 kDa, the previously described molecular weight of centrins. Using isoform specific primers in PCR, the expression of two related but distinct forms of centrin (centrin 1 and centrin 2), can be identified in the retina of human and rat as well as in the mammalian testis, tissues where cilia are present. However, only one isoform (centrin 2) is expressed in nondifferentiated, nonciliated retinal cells (retinoblastoma cells), as well as in rat liver, skeletal muscle, and cardiac muscle. These observations suggest centrin 2 message may be universally expressed while centrin 1 message may be restricted to retina and testis which contain cells that have differentiated cilia or flagella, or their modifications.

Original languageEnglish (US)
Pages (from-to)10-17
Number of pages8
JournalExperimental Cell Research
Volume242
Issue number1
DOIs
StatePublished - Jul 10 1998

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Sulfamethoxazole Drug Combination Trimethoprim
Retina
Protein Isoforms
Cilia
Centrosome
Photoreceptor Connecting Cilium
Testis
Spindle Poles
Basal Bodies
Spindle Apparatus
Flagella
Retinoblastoma
Phosphoproteins
Cellular Structures
Indirect Fluorescent Antibody Technique
Myocardium
Skeletal Muscle
Molecular Weight
Western Blotting
Calcium

Keywords

  • Ca-binding proteins
  • Centrin
  • Cytoskeleton
  • Mammals
  • Photoreceptor cells
  • Retina
  • RT-PCR

ASJC Scopus subject areas

  • Cell Biology

Cite this

Expression of centrin isoforms in the mammalian retina. / Wolfram, Uwe; Salisbury, Jeffrey L.

In: Experimental Cell Research, Vol. 242, No. 1, 10.07.1998, p. 10-17.

Research output: Contribution to journalArticle

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