Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice

S. Cheng, J. Baisch, C. Krco, S. Savarirayan, J. Hanson, K. Hodgson, M. Smart, C. David

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Transgenic mice expressing HLA-DQA1*0301 and HLA-DQB1*0302 genes (DQ8) were produced. The transgenes were then transferred into mouse (Ab(o)) class II negative mice: the only class II molecules expressed in these animals were therefore coded by the HLA-DQ8 genes. Good expression of HLA-DQ molecules was found. Both CD4+ T cells and DQ8-specific T-cell receptor Vβ expressing cells were positively selected in these mice. The HLA-DQ8 molecules expressed in these animals can present various foreign and self antigens and induce T-cell proliferation in vitro. These mice will be invaluable in future studies of the structure and function of HLA-DQ8 genes.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalEuropean Journal of Immunogenetics
Volume23
Issue number1
StatePublished - 1996

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ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Cheng, S., Baisch, J., Krco, C., Savarirayan, S., Hanson, J., Hodgson, K., Smart, M., & David, C. (1996). Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice. European Journal of Immunogenetics, 23(1), 15-20.