Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice

S. Cheng, J. Baisch, C. Krco, S. Savarirayan, J. Hanson, K. Hodgson, M. Smart, C. David

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Transgenic mice expressing HLA-DQA1*0301 and HLA-DQB1*0302 genes (DQ8) were produced. The transgenes were then transferred into mouse (Ab(o)) class II negative mice: the only class II molecules expressed in these animals were therefore coded by the HLA-DQ8 genes. Good expression of HLA-DQ molecules was found. Both CD4+ T cells and DQ8-specific T-cell receptor Vβ expressing cells were positively selected in these mice. The HLA-DQ8 molecules expressed in these animals can present various foreign and self antigens and induce T-cell proliferation in vitro. These mice will be invaluable in future studies of the structure and function of HLA-DQ8 genes.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalEuropean Journal of Immunogenetics
Volume23
Issue number1
StatePublished - 1996

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Transgenic Mice
Genes
HLA-DQ Antigens
T-Lymphocytes
Autoantigens
T-Cell Antigen Receptor
Transgenes
Cell Proliferation
HLA-DQA1 antigen
HLA-DQ8 antigen

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Cheng, S., Baisch, J., Krco, C., Savarirayan, S., Hanson, J., Hodgson, K., ... David, C. (1996). Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice. European Journal of Immunogenetics, 23(1), 15-20.

Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice. / Cheng, S.; Baisch, J.; Krco, C.; Savarirayan, S.; Hanson, J.; Hodgson, K.; Smart, M.; David, C.

In: European Journal of Immunogenetics, Vol. 23, No. 1, 1996, p. 15-20.

Research output: Contribution to journalArticle

Cheng, S, Baisch, J, Krco, C, Savarirayan, S, Hanson, J, Hodgson, K, Smart, M & David, C 1996, 'Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice', European Journal of Immunogenetics, vol. 23, no. 1, pp. 15-20.
Cheng S, Baisch J, Krco C, Savarirayan S, Hanson J, Hodgson K et al. Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice. European Journal of Immunogenetics. 1996;23(1):15-20.
Cheng, S. ; Baisch, J. ; Krco, C. ; Savarirayan, S. ; Hanson, J. ; Hodgson, K. ; Smart, M. ; David, C. / Expression and function of HLA-DQ8 (DQA1*0301/DQB1*0302) genes in transgenic mice. In: European Journal of Immunogenetics. 1996 ; Vol. 23, No. 1. pp. 15-20.
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