TY - JOUR
T1 - Expression and function of a recombinant endothelial nitric oxide synthase gene in porcine coronary arteries
AU - Cable, David G.
AU - O'Brien, Timothy
AU - Kullo, Iftikhar J.
AU - Schwartz, Robert S.
AU - Schaff, Hartzell V.
AU - Pompili, Vincent J.
N1 - Funding Information:
Supported in part by The Mayo Foundation and the Herman C. Krannert Foundation. Performed during D.G.C.’s tenure as a Clinical Investigator Research Fellow.
PY - 1997/9
Y1 - 1997/9
N2 - Objectives: Direct gene transfer of exogenous nitric oxide synthase, with the subsequent increase in nitric oxide production, could represent a potential therapeutic strategy in the treatment of vascular proliferative disorders. The goal of the present study was to determine if porcine coronary arteries could be transduced with an adenoviral vector encoding endothelial nitric oxide synthase (Ad. CMVeNOS) resulting in functional expression. Methods and Results: Segments of porcine right coronary artery were exposed for 1 h at 37°C to either replication-deficient adenovirus encoding bovine endothelial nitric oxide synthase (Ad. CMVeNOS, 5 x 109 pfu/ml) or control adenovirus encoding Escherichia coli β-galactosidase (Ad. CMVLacZ, 5 x 109 pfu/ml). Immunohistochemistry with a monoclonal antibody specific for nitric oxide synthase (NOS) demonstrated recombinant gene expression in both the endothelial and adventitial layers of Ad.CMVeNOS transduced coronaries with only endogenous NOS confirmed in the endothelium of Ad. CMVLacZ arteries. Coronary arteries transduced with Ad.CMVeNOS yielded 517 ± 110 (mean ± S.E.M.) nM/ng nitrite while vessels transduced with Ad.CMVLacZ yielded 126 ± 84 nM/ng (P < 0.05, n = 6). Isometric tension recording, following prostaglandin F(2α) constriction, documented a reduced tension in Ad.CMVeNOS transduced coronaries, compared to matched Ad.CMVLacZ coronaries (6.10 ± 1.0g g vs. 8.45 ± 1.19 g, respectively, P = 0.05, n = 8). This tension differential was eliminated with prior incubation in N(G)-monomethyl-L- arginine (L-NMMA, 10-4 M). The EC50 for calcium ionophore relaxation of Ad.CMVeNOS coronary arteries was reduced compared to Ad. CMVLacZ (- 7.90 ± 0.03 logM vs. - 7.26 ± 0.11 logM, respectively, P < 0.05, n = 8). Conclusions: These studies demonstrate successful transfer of endothelial nitric oxide synthase into porcine coronary arteries as verified by histochemical localization of recombinant protein with an increase of nitric oxide release as demonstrated by enhanced nitrite production and an alteration in vasomotor function.
AB - Objectives: Direct gene transfer of exogenous nitric oxide synthase, with the subsequent increase in nitric oxide production, could represent a potential therapeutic strategy in the treatment of vascular proliferative disorders. The goal of the present study was to determine if porcine coronary arteries could be transduced with an adenoviral vector encoding endothelial nitric oxide synthase (Ad. CMVeNOS) resulting in functional expression. Methods and Results: Segments of porcine right coronary artery were exposed for 1 h at 37°C to either replication-deficient adenovirus encoding bovine endothelial nitric oxide synthase (Ad. CMVeNOS, 5 x 109 pfu/ml) or control adenovirus encoding Escherichia coli β-galactosidase (Ad. CMVLacZ, 5 x 109 pfu/ml). Immunohistochemistry with a monoclonal antibody specific for nitric oxide synthase (NOS) demonstrated recombinant gene expression in both the endothelial and adventitial layers of Ad.CMVeNOS transduced coronaries with only endogenous NOS confirmed in the endothelium of Ad. CMVLacZ arteries. Coronary arteries transduced with Ad.CMVeNOS yielded 517 ± 110 (mean ± S.E.M.) nM/ng nitrite while vessels transduced with Ad.CMVLacZ yielded 126 ± 84 nM/ng (P < 0.05, n = 6). Isometric tension recording, following prostaglandin F(2α) constriction, documented a reduced tension in Ad.CMVeNOS transduced coronaries, compared to matched Ad.CMVLacZ coronaries (6.10 ± 1.0g g vs. 8.45 ± 1.19 g, respectively, P = 0.05, n = 8). This tension differential was eliminated with prior incubation in N(G)-monomethyl-L- arginine (L-NMMA, 10-4 M). The EC50 for calcium ionophore relaxation of Ad.CMVeNOS coronary arteries was reduced compared to Ad. CMVLacZ (- 7.90 ± 0.03 logM vs. - 7.26 ± 0.11 logM, respectively, P < 0.05, n = 8). Conclusions: These studies demonstrate successful transfer of endothelial nitric oxide synthase into porcine coronary arteries as verified by histochemical localization of recombinant protein with an increase of nitric oxide release as demonstrated by enhanced nitrite production and an alteration in vasomotor function.
KW - Adenoviral vector
KW - Gene therapy
KW - Nitric oxide synthase
KW - Porcine coronary artery
KW - Vasomotor function
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U2 - 10.1016/S0008-6363(97)00161-2
DO - 10.1016/S0008-6363(97)00161-2
M3 - Article
C2 - 9415301
AN - SCOPUS:0030729073
SN - 0008-6363
VL - 35
SP - 553
EP - 559
JO - Cardiovascular research
JF - Cardiovascular research
IS - 3
ER -