Experimental design and analysis of antibody microarrays: Applying methods from cDNA arrays

Jeanette E. Eckel-Passow, Antje Hoering, Terry M. Therneau, Irene Ghobrial

Research output: Contribution to journalReview article

39 Scopus citations

Abstract

Protein expression microarrays, also called antibody arrays, represent a new technology that allows the expression level of proteins to be assessed directly. As is also the case with gene expression microarrays, it is hoped that protein expression microarrays will aid in biomarker discovery, predicting disease outcomes and response to treatments, and detecting molecular mechanisms and/or pathways associated with a particular disease state. However, accurately achieving these aims is dependent upon suitable experimental designs, normalization procedures that eliminate systematic bias, and appropriate statistical analyses to assess differential expression or expose expression patterns. In the last five years, a large amount of research has been devoted to two-color cDNA arrays to improve experimental design, normalization and statistical analyses to assess differential expression and classification. These methods are directly applicable to two-color antibody arrays. The objective of this article is to discuss statistical methods that have been developed for cDNA arrays and describe how the methods can be directly applied to antibody arrays.

Original languageEnglish (US)
Pages (from-to)2985-2989
Number of pages5
JournalCancer research
Volume65
Issue number8
DOIs
StatePublished - Apr 15 2005

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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