TY - JOUR
T1 - Experimental design and analysis of antibody microarrays
T2 - Applying methods from cDNA arrays
AU - Eckel-Passow, Jeanette E.
AU - Hoering, Antje
AU - Therneau, Terry M.
AU - Ghobrial, Irene
PY - 2005/4/15
Y1 - 2005/4/15
N2 - Protein expression microarrays, also called antibody arrays, represent a new technology that allows the expression level of proteins to be assessed directly. As is also the case with gene expression microarrays, it is hoped that protein expression microarrays will aid in biomarker discovery, predicting disease outcomes and response to treatments, and detecting molecular mechanisms and/or pathways associated with a particular disease state. However, accurately achieving these aims is dependent upon suitable experimental designs, normalization procedures that eliminate systematic bias, and appropriate statistical analyses to assess differential expression or expose expression patterns. In the last five years, a large amount of research has been devoted to two-color cDNA arrays to improve experimental design, normalization and statistical analyses to assess differential expression and classification. These methods are directly applicable to two-color antibody arrays. The objective of this article is to discuss statistical methods that have been developed for cDNA arrays and describe how the methods can be directly applied to antibody arrays.
AB - Protein expression microarrays, also called antibody arrays, represent a new technology that allows the expression level of proteins to be assessed directly. As is also the case with gene expression microarrays, it is hoped that protein expression microarrays will aid in biomarker discovery, predicting disease outcomes and response to treatments, and detecting molecular mechanisms and/or pathways associated with a particular disease state. However, accurately achieving these aims is dependent upon suitable experimental designs, normalization procedures that eliminate systematic bias, and appropriate statistical analyses to assess differential expression or expose expression patterns. In the last five years, a large amount of research has been devoted to two-color cDNA arrays to improve experimental design, normalization and statistical analyses to assess differential expression and classification. These methods are directly applicable to two-color antibody arrays. The objective of this article is to discuss statistical methods that have been developed for cDNA arrays and describe how the methods can be directly applied to antibody arrays.
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U2 - 10.1158/0008-5472.CAN-04-3213
DO - 10.1158/0008-5472.CAN-04-3213
M3 - Review article
C2 - 15833819
AN - SCOPUS:17144366612
SN - 0008-5472
VL - 65
SP - 2985
EP - 2989
JO - Cancer research
JF - Cancer research
IS - 8
ER -