Evolution of Drug-resistant Acinetobacter baumannii after DCD Renal Transplantation

Hong Jiang, Luxi Cao, Lihui Qu, Tingting Qu, Guangjun Liu, Rending Wang, Bingjue Li, Yuchen Wang, Chaoqun Ying, Miao Chen, Yingying Lu, Shi Feng, Yonghong Xiao, Junwen Wang, Jianyong Wu, Jianghua Chen

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Abstract

Infection after renal transplantation remains a major cause of morbidity and death, especially infection from the extensively drug-resistant bacteria, A. baumannii. A total of fourteen A. baumannii isolates were isolated from the donors' preserved fluid from DCD (donation after cardiac death) renal transplantation and four isolates in the recipients' draining liquid at the Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, from March 2013 to November 2014. An outbreak of A. baumannii emerging after DCD renal transplantation was tracked to understand the transmission of the pathogen. PFGE displayed similar DNA patterns between isolates from the same hospital. Antimicrobial susceptibility tests against thirteen antimicrobial agents were determined using the K-B diffusion method and eTest. Whole-genome sequencing was applied to investigate the genetic relationship of the isolates. With the clinical data and research results, we concluded that the A. baumannii isolates 3R1 and 3R2 was probably transmitted from the donor who acquired the bacteria during his stay in the ICU, while isolate 4R1 was transmitted from 3R1 and 3R2 via medical manipulation. This study demonstrated the value of integration of clinical profiles with molecular methods in outbreak investigation and their importance in controlling infection and preventing serious complications after DCD transplantation.

Original languageEnglish (US)
Article number1968
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Acinetobacter baumannii
Kidney Transplantation
Pharmaceutical Preparations
Disease Outbreaks
Disk Diffusion Antimicrobial Tests
Infection
Hospital Medicine
Bacteria
Infectious Disease Transmission
Kidney Diseases
Heart Transplantation
Anti-Infective Agents
Cause of Death
Genome
Morbidity
DNA
Research

ASJC Scopus subject areas

  • General

Cite this

Evolution of Drug-resistant Acinetobacter baumannii after DCD Renal Transplantation. / Jiang, Hong; Cao, Luxi; Qu, Lihui; Qu, Tingting; Liu, Guangjun; Wang, Rending; Li, Bingjue; Wang, Yuchen; Ying, Chaoqun; Chen, Miao; Lu, Yingying; Feng, Shi; Xiao, Yonghong; Wang, Junwen; Wu, Jianyong; Chen, Jianghua.

In: Scientific Reports, Vol. 7, No. 1, 1968, 01.12.2017.

Research output: Contribution to journalArticle

Jiang, H, Cao, L, Qu, L, Qu, T, Liu, G, Wang, R, Li, B, Wang, Y, Ying, C, Chen, M, Lu, Y, Feng, S, Xiao, Y, Wang, J, Wu, J & Chen, J 2017, 'Evolution of Drug-resistant Acinetobacter baumannii after DCD Renal Transplantation', Scientific Reports, vol. 7, no. 1, 1968. https://doi.org/10.1038/s41598-017-01683-7
Jiang, Hong ; Cao, Luxi ; Qu, Lihui ; Qu, Tingting ; Liu, Guangjun ; Wang, Rending ; Li, Bingjue ; Wang, Yuchen ; Ying, Chaoqun ; Chen, Miao ; Lu, Yingying ; Feng, Shi ; Xiao, Yonghong ; Wang, Junwen ; Wu, Jianyong ; Chen, Jianghua. / Evolution of Drug-resistant Acinetobacter baumannii after DCD Renal Transplantation. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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