Evidence for an androgen receptor in the human placenta

Paula D. McCormick, Anthony J. Razel, Thomas C. Spelsberg, Carolyn B. Coulam

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A steroid-binding protein was examined in human placental cytosol and nuclei which had several characteristics of an androgen receptor. It binds R 1881 (methyltrienolone), a steroid specific for androgen receptors, with high affinity (dissociation constant = 1.3 to 50 nM) and low capacity (n = 1.2 to 21 fmoles/mg of protein). The steroid-binding protein is found in the nuclei as well as the cytosol of the placenta but is not found in either the cytosol or the nuclei of fetal membranes; thus, it is tissue-specific. Among the natural steroids, 5α-dihydrotestosterone competes most efficiently with R 1881 for binding to the protein. Testosterone is the next best competitor. When the ranges of dissociation constants and concentrations of binding sites for placentas of various gestational periods are compared, no significant differences are observed between period of gestation and magnitude of binding constants.

Original languageEnglish (US)
Pages (from-to)8-13
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume140
Issue number1
StatePublished - May 1 1981

Fingerprint

Metribolone
Placenta
Steroids
Cytosol
Carrier Proteins
Androgen Receptors
Extraembryonic Membranes
Dihydrotestosterone
Testosterone
Binding Sites
Pregnancy
human AR protein
Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

McCormick, P. D., Razel, A. J., Spelsberg, T. C., & Coulam, C. B. (1981). Evidence for an androgen receptor in the human placenta. American Journal of Obstetrics and Gynecology, 140(1), 8-13.

Evidence for an androgen receptor in the human placenta. / McCormick, Paula D.; Razel, Anthony J.; Spelsberg, Thomas C.; Coulam, Carolyn B.

In: American Journal of Obstetrics and Gynecology, Vol. 140, No. 1, 01.05.1981, p. 8-13.

Research output: Contribution to journalArticle

McCormick, PD, Razel, AJ, Spelsberg, TC & Coulam, CB 1981, 'Evidence for an androgen receptor in the human placenta', American Journal of Obstetrics and Gynecology, vol. 140, no. 1, pp. 8-13.
McCormick PD, Razel AJ, Spelsberg TC, Coulam CB. Evidence for an androgen receptor in the human placenta. American Journal of Obstetrics and Gynecology. 1981 May 1;140(1):8-13.
McCormick, Paula D. ; Razel, Anthony J. ; Spelsberg, Thomas C. ; Coulam, Carolyn B. / Evidence for an androgen receptor in the human placenta. In: American Journal of Obstetrics and Gynecology. 1981 ; Vol. 140, No. 1. pp. 8-13.
@article{ba758af03e94449984e8a5338832ed52,
title = "Evidence for an androgen receptor in the human placenta",
abstract = "A steroid-binding protein was examined in human placental cytosol and nuclei which had several characteristics of an androgen receptor. It binds R 1881 (methyltrienolone), a steroid specific for androgen receptors, with high affinity (dissociation constant = 1.3 to 50 nM) and low capacity (n = 1.2 to 21 fmoles/mg of protein). The steroid-binding protein is found in the nuclei as well as the cytosol of the placenta but is not found in either the cytosol or the nuclei of fetal membranes; thus, it is tissue-specific. Among the natural steroids, 5α-dihydrotestosterone competes most efficiently with R 1881 for binding to the protein. Testosterone is the next best competitor. When the ranges of dissociation constants and concentrations of binding sites for placentas of various gestational periods are compared, no significant differences are observed between period of gestation and magnitude of binding constants.",
author = "McCormick, {Paula D.} and Razel, {Anthony J.} and Spelsberg, {Thomas C.} and Coulam, {Carolyn B.}",
year = "1981",
month = "5",
day = "1",
language = "English (US)",
volume = "140",
pages = "8--13",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "1",

}

TY - JOUR

T1 - Evidence for an androgen receptor in the human placenta

AU - McCormick, Paula D.

AU - Razel, Anthony J.

AU - Spelsberg, Thomas C.

AU - Coulam, Carolyn B.

PY - 1981/5/1

Y1 - 1981/5/1

N2 - A steroid-binding protein was examined in human placental cytosol and nuclei which had several characteristics of an androgen receptor. It binds R 1881 (methyltrienolone), a steroid specific for androgen receptors, with high affinity (dissociation constant = 1.3 to 50 nM) and low capacity (n = 1.2 to 21 fmoles/mg of protein). The steroid-binding protein is found in the nuclei as well as the cytosol of the placenta but is not found in either the cytosol or the nuclei of fetal membranes; thus, it is tissue-specific. Among the natural steroids, 5α-dihydrotestosterone competes most efficiently with R 1881 for binding to the protein. Testosterone is the next best competitor. When the ranges of dissociation constants and concentrations of binding sites for placentas of various gestational periods are compared, no significant differences are observed between period of gestation and magnitude of binding constants.

AB - A steroid-binding protein was examined in human placental cytosol and nuclei which had several characteristics of an androgen receptor. It binds R 1881 (methyltrienolone), a steroid specific for androgen receptors, with high affinity (dissociation constant = 1.3 to 50 nM) and low capacity (n = 1.2 to 21 fmoles/mg of protein). The steroid-binding protein is found in the nuclei as well as the cytosol of the placenta but is not found in either the cytosol or the nuclei of fetal membranes; thus, it is tissue-specific. Among the natural steroids, 5α-dihydrotestosterone competes most efficiently with R 1881 for binding to the protein. Testosterone is the next best competitor. When the ranges of dissociation constants and concentrations of binding sites for placentas of various gestational periods are compared, no significant differences are observed between period of gestation and magnitude of binding constants.

UR - http://www.scopus.com/inward/record.url?scp=0019405549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019405549&partnerID=8YFLogxK

M3 - Article

VL - 140

SP - 8

EP - 13

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 1

ER -