Establishment, maintenance and in vitro and in vivo applications of primary human glioblastoma multiforme (GBM) xenograft models for translational biology studies and drug discovery.

Brett L. Carlson, Jenny L. Pokorny, Mark A. Schroeder, Jann N Sarkaria

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Abstract

Development of clinically relevant tumor model systems for glioblastoma multiforme (GBM) is important for advancement of basic and translational biology. One model that has gained wide acceptance in the neuro-oncology community is the primary xenograft model. This model entails the engraftment of patient tumor specimens into the flank of nude mice and subsequent serial passage of these tumors in the flank of mice. These tumors are then used to establish short-term explant cultures or intracranial xenografts. This unit describes detailed procedures for establishment, maintenance, and utilization of a primary GBM xenograft panel for the purpose of using them as tumor models for basic or translational studies.

Original languageEnglish (US)
JournalCurrent protocols in pharmacology / editorial board, S.J. Enna (editor-in-chief) ... [et al.]
VolumeChapter 14
StatePublished - Mar 2011

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Drug Discovery
Glioblastoma
Heterografts
Maintenance
Neoplasms
Serial Passage
Nude Mice
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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