Essential role for CAML in follicular B cell survival and homeostasis

Nicholas A. Zane, Justin H. Gundelach, Lonn D. Lindquist, Richard J Bram

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Calcium-modulating cyclophilin ligand (CAML) is a ubiquitously expressed protein that is important during thymopoiesis. However, whether it serves a function in mature lymphocytes is unknown. In this article, we show that CAML is essential for survival of peripheral follicular (Fo) B cells. Conditional deletion of CAML in CD19-Cre transgenic mice caused a significant reduction in Fo cell numbers and increased rates of homeostatic proliferation. CAML-deficient Fo cells showed increased cellular turnover and normal proliferative ability. Although CAML-deficient Fo cells responded to AgR stimulation and to B cell activating factor, they displayed decreased survival and increased apoptosis following stimulation with LPS and IL-4 in vitro. Failure to survive was not due to aberrant B cell development in the absence of CAML, because induced deletion of the gene in mature cells resulted in a similar phenotype. These data establish an essential and ongoing role for CAML in the long-term survival of mature B cells.

Original languageEnglish (US)
Pages (from-to)3009-3018
Number of pages10
JournalJournal of Immunology
Volume188
Issue number7
DOIs
StatePublished - Apr 1 2012

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Cyclophilins
Cell Survival
Homeostasis
B-Lymphocytes
Ligands
Calcium
B-Cell Activating Factor
Gene Deletion
Interleukin-4
Transgenic Mice
Cell Count
Lymphocytes
Apoptosis
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Essential role for CAML in follicular B cell survival and homeostasis. / Zane, Nicholas A.; Gundelach, Justin H.; Lindquist, Lonn D.; Bram, Richard J.

In: Journal of Immunology, Vol. 188, No. 7, 01.04.2012, p. 3009-3018.

Research output: Contribution to journalArticle

Zane, Nicholas A. ; Gundelach, Justin H. ; Lindquist, Lonn D. ; Bram, Richard J. / Essential role for CAML in follicular B cell survival and homeostasis. In: Journal of Immunology. 2012 ; Vol. 188, No. 7. pp. 3009-3018.
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