Epstein-Barr Virus MicroRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival

Alessandra Ferrajoli, Cristina Ivan, Maria Ciccone, Masayoshi Shimizu, Yoshiaki Kita, Masahisha Ohtsuka, Lucilla D'Abundo, Jun Qiang, Susan Lerner, Nazila Nouraee, Kari G. Rabe, Laura Z. Rassenti, Katrien Van Roosbroeck, John T. Manning, Yuan Yuan, Xinna Zhang, Tait D. Shanafelt, William G. Wierda, Silvia Sabbioni, Jeffrey J. Tarrand & 8 others Zeev Estrov, Milan Radovich, Han Liang, Massimo Negrini, Thomas J. Kipps, Neil Elliot Kay, Michael Keating, George A. Calin

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Although numerous studies highlighted the role of Epstein-Barr Virus (EBV) in B-cell transformation, the involvement of EBV proteins or genome in the development of the most frequent adult leukemia, chronic lymphocytic leukemia (CLL), has not yet been defined. We hypothesized that EBV microRNAs contribute to progression of CLL and demonstrated the presence of EBV miRNAs in B-cells, in paraffin-embedded bone marrow biopsies and in the plasma of patients with CLL by using three different methods (small RNA-sequencing, quantitative reverse transcription PCR [q-RT-PCR] and miRNAs in situ hybridization [miRNA-ISH]). We found that EBV miRNA BHRF1-1 expression levels were significantly higher in the plasma of patients with CLL compared with healthy individuals (p.

Original languageEnglish (US)
Pages (from-to)572-582
Number of pages11
JournalEBioMedicine
Volume2
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
MicroRNAs
Human Herpesvirus 4
Viruses
Survival
B-Lymphocytes
Cells
RNA Sequence Analysis
Plasmas
Biopsy
Transcription
Paraffin
Reverse Transcription
In Situ Hybridization
Bone
Leukemia
Genes
Bone Marrow
Genome
RNA

Keywords

  • BHRF1-1
  • Chronic lymphocytic leukemia
  • Epstein-Barr Virus
  • MiRNAs
  • Overall survival

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Epstein-Barr Virus MicroRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival. / Ferrajoli, Alessandra; Ivan, Cristina; Ciccone, Maria; Shimizu, Masayoshi; Kita, Yoshiaki; Ohtsuka, Masahisha; D'Abundo, Lucilla; Qiang, Jun; Lerner, Susan; Nouraee, Nazila; Rabe, Kari G.; Rassenti, Laura Z.; Van Roosbroeck, Katrien; Manning, John T.; Yuan, Yuan; Zhang, Xinna; Shanafelt, Tait D.; Wierda, William G.; Sabbioni, Silvia; Tarrand, Jeffrey J.; Estrov, Zeev; Radovich, Milan; Liang, Han; Negrini, Massimo; Kipps, Thomas J.; Kay, Neil Elliot; Keating, Michael; Calin, George A.

In: EBioMedicine, Vol. 2, No. 6, 01.06.2015, p. 572-582.

Research output: Contribution to journalArticle

Ferrajoli, A, Ivan, C, Ciccone, M, Shimizu, M, Kita, Y, Ohtsuka, M, D'Abundo, L, Qiang, J, Lerner, S, Nouraee, N, Rabe, KG, Rassenti, LZ, Van Roosbroeck, K, Manning, JT, Yuan, Y, Zhang, X, Shanafelt, TD, Wierda, WG, Sabbioni, S, Tarrand, JJ, Estrov, Z, Radovich, M, Liang, H, Negrini, M, Kipps, TJ, Kay, NE, Keating, M & Calin, GA 2015, 'Epstein-Barr Virus MicroRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival', EBioMedicine, vol. 2, no. 6, pp. 572-582. https://doi.org/10.1016/j.ebiom.2015.04.018
Ferrajoli, Alessandra ; Ivan, Cristina ; Ciccone, Maria ; Shimizu, Masayoshi ; Kita, Yoshiaki ; Ohtsuka, Masahisha ; D'Abundo, Lucilla ; Qiang, Jun ; Lerner, Susan ; Nouraee, Nazila ; Rabe, Kari G. ; Rassenti, Laura Z. ; Van Roosbroeck, Katrien ; Manning, John T. ; Yuan, Yuan ; Zhang, Xinna ; Shanafelt, Tait D. ; Wierda, William G. ; Sabbioni, Silvia ; Tarrand, Jeffrey J. ; Estrov, Zeev ; Radovich, Milan ; Liang, Han ; Negrini, Massimo ; Kipps, Thomas J. ; Kay, Neil Elliot ; Keating, Michael ; Calin, George A. / Epstein-Barr Virus MicroRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival. In: EBioMedicine. 2015 ; Vol. 2, No. 6. pp. 572-582.
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AU - Kita, Yoshiaki

AU - Ohtsuka, Masahisha

AU - D'Abundo, Lucilla

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AU - Sabbioni, Silvia

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AU - Liang, Han

AU - Negrini, Massimo

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