Epidermal growth factor receptor (EGFR) signaling regulates epiphyseal cartilage development through β-catenin-dependent and -independent pathways

Xianrong Zhang, Ji Zhu, Yumei Li, Tiao Lin, Valerie A. Siclari, Abhishek Chandra, Elena M. Candela, Eiki Koyama, Motomi Enomoto-Iwamoto, Ling Qin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The epidermal growth factor receptor (EGFR) is an essential player in the development of multiple organs during embryonic and postnatal stages. To understand its role in epiphyseal cartilage development, we generated transgenic mice with conditionally inactivated EGFR in chondrocytes. Postnatally, these mice exhibited a normal initiation of cartilage canals at the perichondrium, but the excavation of these canals into the cartilage was strongly suppressed, resulting in a delay in the formation of the secondary ossification center (SOC). This delay was accompanied by normal chondrocyte hypertrophy but decreased mineralization and apoptosis of hypertrophic chondrocytes and reduced osteoclast number at the border of marrow space. Immunohistochemical analyses demonstrated that inactivation of chondrocyte-specific EGFR signaling reduced the amounts of matrix metalloproteinases (MMP9, -13, and -14) and RANKL (receptor activator of NF-κB ligand) in the hypertrophic chondrocytes close to the marrow space and decreased the cartilage matrix degradation in the SOC. Analyses of EGFR downstream signaling pathways in primary epiphyseal chondrocytes revealed that up-regulation of MMP9 and RANKL by EGFR signaling was partially mediated by the canonical Wnt/ β- catenin pathway, whereas EGFR-enhanced MMP13 expression was not. Further biochemical studies suggested that EGFR signaling stimulates the phosphorylation of LRP6, increases active β-catenin level, and induces its nuclear translocation. In line with these in vitro studies, deficiency in chondrocyte-specific EGFR activity reduced β-catenin amount in hypertrophic chondrocytes in vivo. In conclusion, our work demonstrates that chondrocyte-specific EGFR signaling is an important regulator of cartilage matrix degradation during SOC formation and epiphyseal cartilage development and that its actions are partially mediated by activating the β-catenin pathway.

Original languageEnglish (US)
Pages (from-to)32229-32240
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number45
DOIs
StatePublished - Nov 8 2013
Externally publishedYes

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Catenins
Growth Plate
Cartilage
Chondrocytes
Epidermal Growth Factor Receptor
Osteogenesis
Canals
Bone Marrow
Matrix Metalloproteinase 13
Degradation
Phosphorylation
Wnt Signaling Pathway
Osteoclasts
Matrix Metalloproteinases
Excavation
Hypertrophy
Transgenic Mice
Up-Regulation
Apoptosis
Ligands

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Epidermal growth factor receptor (EGFR) signaling regulates epiphyseal cartilage development through β-catenin-dependent and -independent pathways. / Zhang, Xianrong; Zhu, Ji; Li, Yumei; Lin, Tiao; Siclari, Valerie A.; Chandra, Abhishek; Candela, Elena M.; Koyama, Eiki; Enomoto-Iwamoto, Motomi; Qin, Ling.

In: Journal of Biological Chemistry, Vol. 288, No. 45, 08.11.2013, p. 32229-32240.

Research output: Contribution to journalArticle

Zhang, Xianrong ; Zhu, Ji ; Li, Yumei ; Lin, Tiao ; Siclari, Valerie A. ; Chandra, Abhishek ; Candela, Elena M. ; Koyama, Eiki ; Enomoto-Iwamoto, Motomi ; Qin, Ling. / Epidermal growth factor receptor (EGFR) signaling regulates epiphyseal cartilage development through β-catenin-dependent and -independent pathways. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 45. pp. 32229-32240.
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