Eosinophil Peroxidase Staining Enhances the Diagnostic Utility of the Cytosponge in Eosinophilic Esophagitis

Mia Y. Masuda; Suzanne M. Barshow; Shipra Garg; Arina Putikova; William E. LeSuer; Jeffrey A. Alexander; David A Katzka; Evan S. Dellon; Hirohito Kita; Jennifer L. Horsley-Silva; Alfred D. Doyle; Benjamin L. Wright

doi:10.14309/ctg.0000000000000534

Eosinophil Peroxidase Staining Enhances the Diagnostic Utility of the Cytosponge in Eosinophilic Esophagitis

Mia Y. Masuda, Suzanne M. Barshow, Shipra Garg, Arina Putikova, William E. LeSuer, Jeffrey A. Alexander, David A Katzka, Evan S. Dellon, Hirohito Kita, Jennifer L. Horsley-Silva, Alfred D. Doyle, Benjamin L. Wright

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Abstract

INTRODUCTION: We aimed to assess the diagnostic utility of eosinophil peroxidase (EPX) staining on Cytosponge (CS) samples in eosinophilic esophagitis (EoE). METHODS: Esophageal biopsy (BX) samples from adult subjects with EoE were assessed using peak eosinophils per high-power field (eos/hpf), EPX, and the EoE histologic scoring system. EPX staining and eos/hpf were compared (BX vs CS). RESULTS: CS EPX positivity correlated with eos/hpf (CS [ r = 0.82, P < 0.0001]; BX [ r = 0.65, P < 0.0001]) and EoE histologic scoring system (grade [ r = 0.62, P < 0.00001]; stage [ r = 0.61, P < 0.0001]). CS EPX identified subjects with active EoE (area under the curve = 0.86, P < 0.0001). DISCUSSION: The correlation of CS EPX with eosinophilic inflammation and histologic disease severity supports its diagnostic utility in EoE.

Original language	English (US)
Pages (from-to)	e00534
Journal	Clinical and translational gastroenterology
Volume	13
Issue number	11
DOIs	https://doi.org/10.14309/ctg.0000000000000534
State	Published - Nov 1 2022

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Gastroenterology

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Masuda, M. Y., Barshow, S. M., Garg, S., Putikova, A., LeSuer, W. E., Alexander, J. A., Katzka, D. A., Dellon, E. S., Kita, H., Horsley-Silva, J. L., Doyle, A. D., & Wright, B. L. (2022). Eosinophil Peroxidase Staining Enhances the Diagnostic Utility of the Cytosponge in Eosinophilic Esophagitis. Clinical and translational gastroenterology, 13(11), e00534. https://doi.org/10.14309/ctg.0000000000000534

Masuda, MY, Barshow, SM, Garg, S, Putikova, A, LeSuer, WE, Alexander, JA, Katzka, DA, Dellon, ES, Kita, H, Horsley-Silva, JL, Doyle, AD & Wright, BL 2022, 'Eosinophil Peroxidase Staining Enhances the Diagnostic Utility of the Cytosponge in Eosinophilic Esophagitis', Clinical and translational gastroenterology, vol. 13, no. 11, pp. e00534. https://doi.org/10.14309/ctg.0000000000000534

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title = "Eosinophil Peroxidase Staining Enhances the Diagnostic Utility of the Cytosponge in Eosinophilic Esophagitis",

abstract = "INTRODUCTION: We aimed to assess the diagnostic utility of eosinophil peroxidase (EPX) staining on Cytosponge (CS) samples in eosinophilic esophagitis (EoE). METHODS: Esophageal biopsy (BX) samples from adult subjects with EoE were assessed using peak eosinophils per high-power field (eos/hpf), EPX, and the EoE histologic scoring system. EPX staining and eos/hpf were compared (BX vs CS). RESULTS: CS EPX positivity correlated with eos/hpf (CS [ r = 0.82, P < 0.0001]; BX [ r = 0.65, P < 0.0001]) and EoE histologic scoring system (grade [ r = 0.62, P < 0.00001]; stage [ r = 0.61, P < 0.0001]). CS EPX identified subjects with active EoE (area under the curve = 0.86, P < 0.0001). DISCUSSION: The correlation of CS EPX with eosinophilic inflammation and histologic disease severity supports its diagnostic utility in EoE.",

author = "Masuda, {Mia Y.} and Barshow, {Suzanne M.} and Shipra Garg and Arina Putikova and LeSuer, {William E.} and Alexander, {Jeffrey A.} and Katzka, {David A} and Dellon, {Evan S.} and Hirohito Kita and Horsley-Silva, {Jennifer L.} and Doyle, {Alfred D.} and Wright, {Benjamin L.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2022 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology.",

year = "2022",

month = nov,

day = "1",

doi = "10.14309/ctg.0000000000000534",

language = "English (US)",

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T1 - Eosinophil Peroxidase Staining Enhances the Diagnostic Utility of the Cytosponge in Eosinophilic Esophagitis

AU - Masuda, Mia Y.

AU - Barshow, Suzanne M.

AU - Garg, Shipra

AU - Putikova, Arina

AU - LeSuer, William E.

AU - Alexander, Jeffrey A.

AU - Katzka, David A

AU - Dellon, Evan S.

AU - Kita, Hirohito

AU - Horsley-Silva, Jennifer L.

AU - Doyle, Alfred D.

AU - Wright, Benjamin L.

PY - 2022/11/1

Y1 - 2022/11/1

N2 - INTRODUCTION: We aimed to assess the diagnostic utility of eosinophil peroxidase (EPX) staining on Cytosponge (CS) samples in eosinophilic esophagitis (EoE). METHODS: Esophageal biopsy (BX) samples from adult subjects with EoE were assessed using peak eosinophils per high-power field (eos/hpf), EPX, and the EoE histologic scoring system. EPX staining and eos/hpf were compared (BX vs CS). RESULTS: CS EPX positivity correlated with eos/hpf (CS [ r = 0.82, P < 0.0001]; BX [ r = 0.65, P < 0.0001]) and EoE histologic scoring system (grade [ r = 0.62, P < 0.00001]; stage [ r = 0.61, P < 0.0001]). CS EPX identified subjects with active EoE (area under the curve = 0.86, P < 0.0001). DISCUSSION: The correlation of CS EPX with eosinophilic inflammation and histologic disease severity supports its diagnostic utility in EoE.

AB - INTRODUCTION: We aimed to assess the diagnostic utility of eosinophil peroxidase (EPX) staining on Cytosponge (CS) samples in eosinophilic esophagitis (EoE). METHODS: Esophageal biopsy (BX) samples from adult subjects with EoE were assessed using peak eosinophils per high-power field (eos/hpf), EPX, and the EoE histologic scoring system. EPX staining and eos/hpf were compared (BX vs CS). RESULTS: CS EPX positivity correlated with eos/hpf (CS [ r = 0.82, P < 0.0001]; BX [ r = 0.65, P < 0.0001]) and EoE histologic scoring system (grade [ r = 0.62, P < 0.00001]; stage [ r = 0.61, P < 0.0001]). CS EPX identified subjects with active EoE (area under the curve = 0.86, P < 0.0001). DISCUSSION: The correlation of CS EPX with eosinophilic inflammation and histologic disease severity supports its diagnostic utility in EoE.

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Eosinophil Peroxidase Staining Enhances the Diagnostic Utility of the Cytosponge in Eosinophilic Esophagitis

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