Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma

K. L. Denzler, S. C. Farmer, J. R. Crosby, M. Borchers, G. Cieslewicz, K. A. Larson, S. Cormier-Regard, N. A. Lee, J. J. Lee

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

The relationship between eosinophils and the development of Ag-induced pulmonary pathologies, including airway hyper-responsiveness, was investigated using mice deficient for the secondary granule component, major basic protein-1 (mMBP-1). The loss of mMBP-1 had no effect on OVA-induced airway histopathologies or inflammatory cell recruitment. Lung function measurements of knockout mice demonstrated a generalized hyporeactivity to methacholine-induced airflow changes (relative to wild type); however, this baseline phenotype was observable only with methacholine; no relative airflow changes were observed in response to another nonspecific stimulus (serotonin). Moreover, OVA sensitization/aerosol challenge of wild-type and mMBP-1(-/-) mice resulted in identical dose-response changes to either methacholine or serotonin. Thus, the airway hyper-responsiveness in murine models of asthma occurs in the absence of mMBP-1.

Original languageEnglish (US)
Pages (from-to)5509-5517
Number of pages9
JournalJournal of Immunology
Volume165
Issue number10
DOIs
StatePublished - Nov 15 2000

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Denzler, K. L., Farmer, S. C., Crosby, J. R., Borchers, M., Cieslewicz, G., Larson, K. A., Cormier-Regard, S., Lee, N. A., & Lee, J. J. (2000). Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. Journal of Immunology, 165(10), 5509-5517. https://doi.org/10.4049/jimmunol.165.10.5509