Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma

K. L. Denzler, S. C. Farmer, J. R. Crosby, M. Borchers, G. Cieslewicz, K. A. Larson, S. Cormier-Regard, Nancy A Lee, J. J. Lee

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Abstract

The relationship between eosinophils and the development of Ag-induced pulmonary pathologies, including airway hyper-responsiveness, was investigated using mice deficient for the secondary granule component, major basic protein-1 (mMBP-1). The loss of mMBP-1 had no effect on OVA-induced airway histopathologies or inflammatory cell recruitment. Lung function measurements of knockout mice demonstrated a generalized hyporeactivity to methacholine-induced airflow changes (relative to wild type); however, this baseline phenotype was observable only with methacholine; no relative airflow changes were observed in response to another nonspecific stimulus (serotonin). Moreover, OVA sensitization/aerosol challenge of wild-type and mMBP-1(-/-) mice resulted in identical dose-response changes to either methacholine or serotonin. Thus, the airway hyper-responsiveness in murine models of asthma occurs in the absence of mMBP-1.

Original languageEnglish (US)
Pages (from-to)5509-5517
Number of pages9
JournalJournal of Immunology
Volume165
Issue number10
StatePublished - Nov 15 2000

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Eosinophil Major Basic Protein
Methacholine Chloride
Respiratory Hypersensitivity
Allergens
Asthma
Pathology
Serotonin
Lung
Aerosols
Eosinophils
Knockout Mice
Phenotype
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Denzler, K. L., Farmer, S. C., Crosby, J. R., Borchers, M., Cieslewicz, G., Larson, K. A., ... Lee, J. J. (2000). Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. Journal of Immunology, 165(10), 5509-5517.

Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. / Denzler, K. L.; Farmer, S. C.; Crosby, J. R.; Borchers, M.; Cieslewicz, G.; Larson, K. A.; Cormier-Regard, S.; Lee, Nancy A; Lee, J. J.

In: Journal of Immunology, Vol. 165, No. 10, 15.11.2000, p. 5509-5517.

Research output: Contribution to journalArticle

Denzler, KL, Farmer, SC, Crosby, JR, Borchers, M, Cieslewicz, G, Larson, KA, Cormier-Regard, S, Lee, NA & Lee, JJ 2000, 'Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma', Journal of Immunology, vol. 165, no. 10, pp. 5509-5517.
Denzler KL, Farmer SC, Crosby JR, Borchers M, Cieslewicz G, Larson KA et al. Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. Journal of Immunology. 2000 Nov 15;165(10):5509-5517.
Denzler, K. L. ; Farmer, S. C. ; Crosby, J. R. ; Borchers, M. ; Cieslewicz, G. ; Larson, K. A. ; Cormier-Regard, S. ; Lee, Nancy A ; Lee, J. J. / Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. In: Journal of Immunology. 2000 ; Vol. 165, No. 10. pp. 5509-5517.
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