Eosinophil differentiation of human umbilical cord mononuclear cells and prolonged survival of mature eosinophils by murine EL-4 thymoma cell conditioned medium

Rosa M. Ten, Joseph H. Butterfield, Hirohito Kita, Deborah A. Weiler, Steven Fischkoff, Teruko Ishizaka, Colin J. Sanderson, Gerald J. Gleich

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Umbilical cord mononuclear cells, HL-60 cells, HL-60 clones selected for eosinophil differentiation, and the eosinophil leukemia cell line EoL were tested for their ability to produce eosinophil peroxidase. HL-60 clones selected for eosinophil differentiation produced eosinophil peroxidase, as judged by staining of cells for cyanide-resistant peroxidase activity; however, these cells lost their ability to produce eosinophil peroxidase in long-term culture. In contrast, eosinophil precursors from human umbilical cord blood mononuclear cells stimulated with murine EL-4 conditioned medium (EL-4 CM) were regularly induced to eosinophil protein synthesis, including eosinophil peroxidase, major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin, as assessed by cyanide-resistant peroxidase and immunofluorescence staining. This induction by EL-4 CM is either at the level of gene transcription or mRNA stabilization, as shown by the increase of total mRNA for eosinophil peroxidase, major basic protein, and eosinophil-derived neurotoxin by Northern blot analyses. Purified peripheral blood eosinophils incubated for 4 days with EL-4 CM had increased survival over control eosinophils. Moreover, this enhanced survival was specifically blocked by antiserum to interleukin 5. Our results suggest that the effects of EL-4 CM on human umbilical cord mononuclear cells and mature eosinophils are due to the presence of interleukin 5.

Original languageEnglish (US)
Pages (from-to)350-359
Number of pages10
JournalCytokine
Volume3
Issue number4
DOIs
StatePublished - 1991

Fingerprint

Eosinophil Peroxidase
Thymoma
Umbilical Cord
Conditioned Culture Medium
Eosinophils
Cell Survival
Eosinophil-Derived Neurotoxin
Interleukin-5
Cyanides
Eosinophil Major Basic Protein
Peroxidase
Blood
Eosinophil Cationic Protein
Proteins
Messenger RNA
Transcription
Clone Cells
Staining and Labeling
Immune Sera
Stabilization

Keywords

  • EL-4
  • eosinophil peroxidase
  • eosinophils
  • interleukin 5
  • umbilical cord mononuclear cells

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Eosinophil differentiation of human umbilical cord mononuclear cells and prolonged survival of mature eosinophils by murine EL-4 thymoma cell conditioned medium. / Ten, Rosa M.; Butterfield, Joseph H.; Kita, Hirohito; Weiler, Deborah A.; Fischkoff, Steven; Ishizaka, Teruko; Sanderson, Colin J.; Gleich, Gerald J.

In: Cytokine, Vol. 3, No. 4, 1991, p. 350-359.

Research output: Contribution to journalArticle

Ten, Rosa M. ; Butterfield, Joseph H. ; Kita, Hirohito ; Weiler, Deborah A. ; Fischkoff, Steven ; Ishizaka, Teruko ; Sanderson, Colin J. ; Gleich, Gerald J. / Eosinophil differentiation of human umbilical cord mononuclear cells and prolonged survival of mature eosinophils by murine EL-4 thymoma cell conditioned medium. In: Cytokine. 1991 ; Vol. 3, No. 4. pp. 350-359.
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