Enhanced endothelial progenitor cell angiogenic potency, present in early experimental renovascular hypertension, deteriorates with disease duration

Xiang Yang Zhu, Victor H Urbieta Caceres, Frederic D. Favreau, James D. Krier, Amir Lerman, Lilach O Lerman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Endothelial progenitor cells (EPCs) augment neovascularization and repair of damaged tissues, but may undergo functional changes during exposure to cardiovascular risk factors. This study tested the hypothesis that early renovascular hypertension (RVH) modulates the temporal pattern of EPC function that deteriorates with disease duration. Methods: RVH was induced in domestic pigs by unilateral renal artery stenosis. EPCs were cultured after 3, 6, and 12 weeks of RVH or normal control to evaluate EPC function, growth factor, and homing receptor expression. Plasma renin activity (PRA), vascular endothelial growth factor (VEGF), and its soluble receptor-1 (sFlt-1) were measured in plasma. EPCs (10×10 6) isolated from 3-week RVH or from normal pigs were also injected into control kidneys (n=6-7, each group), and 4 weeks later single-kidney renal blood flow (RBF) and glomerular filtration rate (GFR) were evaluated. Microvascular density was studied ex vivo using microcomputed tomography. Results: Blood pressure peaked at 3 weeks and remained higher than normal throughout the study. Systemic PRA also peaked after 3 weeks of RVH and declined thereafter, whereas sFlt-1 showed a reciprocal pattern. In vivo, only RVH but not normal EPCs increased RBF, GFR, and microvascular density. RVH-EPCs showed in vitro enhanced proliferation, tube formation, VEGF, and homing receptor expression that peaked at 3 weeks, which were abolished by valsartan and returned to baseline levels after 12 weeks of RVH. EPC number remained unchanged throughout the study. Conclusion: A transient enhancement of EPC function, mediated by angiotensin II, may contribute to compensatory vascular adaptation in early RVH, but is lost as hypertension persists.

Original languageEnglish (US)
Pages (from-to)1972-1979
Number of pages8
JournalJournal of Hypertension
Volume29
Issue number10
DOIs
StatePublished - Oct 2011

Fingerprint

Renovascular Hypertension
Renal Circulation
Valsartan
Glomerular Filtration Rate
Renin
Kidney
Endothelial Progenitor Cells
X-Ray Microtomography
Sus scrofa
Renal Artery Obstruction
Vascular Endothelial Growth Factor Receptor
Growth Factor Receptors
Angiotensin II
Vascular Endothelial Growth Factor A
Blood Vessels
Swine
Cell Count
Blood Pressure
Hypertension

Keywords

  • Angiogenesis
  • Angiotensin II
  • Endothelial progenitor cell
  • Growth factor
  • Renal hypertension

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Enhanced endothelial progenitor cell angiogenic potency, present in early experimental renovascular hypertension, deteriorates with disease duration. / Zhu, Xiang Yang; Caceres, Victor H Urbieta; Favreau, Frederic D.; Krier, James D.; Lerman, Amir; Lerman, Lilach O.

In: Journal of Hypertension, Vol. 29, No. 10, 10.2011, p. 1972-1979.

Research output: Contribution to journalArticle

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abstract = "Background: Endothelial progenitor cells (EPCs) augment neovascularization and repair of damaged tissues, but may undergo functional changes during exposure to cardiovascular risk factors. This study tested the hypothesis that early renovascular hypertension (RVH) modulates the temporal pattern of EPC function that deteriorates with disease duration. Methods: RVH was induced in domestic pigs by unilateral renal artery stenosis. EPCs were cultured after 3, 6, and 12 weeks of RVH or normal control to evaluate EPC function, growth factor, and homing receptor expression. Plasma renin activity (PRA), vascular endothelial growth factor (VEGF), and its soluble receptor-1 (sFlt-1) were measured in plasma. EPCs (10×10 6) isolated from 3-week RVH or from normal pigs were also injected into control kidneys (n=6-7, each group), and 4 weeks later single-kidney renal blood flow (RBF) and glomerular filtration rate (GFR) were evaluated. Microvascular density was studied ex vivo using microcomputed tomography. Results: Blood pressure peaked at 3 weeks and remained higher than normal throughout the study. Systemic PRA also peaked after 3 weeks of RVH and declined thereafter, whereas sFlt-1 showed a reciprocal pattern. In vivo, only RVH but not normal EPCs increased RBF, GFR, and microvascular density. RVH-EPCs showed in vitro enhanced proliferation, tube formation, VEGF, and homing receptor expression that peaked at 3 weeks, which were abolished by valsartan and returned to baseline levels after 12 weeks of RVH. EPC number remained unchanged throughout the study. Conclusion: A transient enhancement of EPC function, mediated by angiotensin II, may contribute to compensatory vascular adaptation in early RVH, but is lost as hypertension persists.",
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AU - Zhu, Xiang Yang

AU - Caceres, Victor H Urbieta

AU - Favreau, Frederic D.

AU - Krier, James D.

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KW - Growth factor

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