Enhanced coronary vasoconstriction to endothelin-B-receptor activation in experimental congestive heart failure

Charles R. Cannan, John C. Burnett, Amir Lerman

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Background: Endothelin (ET), a coronary vasoconstrictor, mediates its activity through the specific receptors ET-A and ET-B, which may demonstrate different activity under pathophysiological conditions. Thoracic inferior vena cava constriction (TIVCC) is an experimental model of congestive heart failure that is characterized by a decrease in cardiac output and an increase in circulating ET concentrations. The present study was designed to test the hypothesis that experimental heart failure altered coronary vascular responsiveness to ET-A- and ET-B-receptor stimulation in vivo. Methods and Results: ET-1 was infused at a rate of 2 ng/kg per minute into the left circumflex coronary artery in normal dogs (n=5) and in dogs subjected to TIVCC (TIVCC dogs, n=6). Similarly, sarafotoxin, an ET-B-receptor agonist, was infused at the same dosage in normal (n=5) and TIVCC (n=6) dogs. Intracoronary infusion of ET-1 significantly decreased coronary blood flow and increased coronary vascular resistance in normal dogs: this effect was significantly attenuated in TIVCC compared with normal dogs. The percent changes in coronary blood flow and coronary vascular resistance in the TIVCC compared with the normal dogs was -11±8% versus -48±7% (P<.01) and 20±10% versus 105±23% (P<.01), respectively. There wits no significant effect on coronary blood flow, coronary vascular resistance, or coronary artery diameter in normal dogs that received an intracoronary infusion of sarafotoxin. In contrast, the administration of intracoronary sarafotoxin in TIVCC compared with normal dogs resulted in significant percent changes in coronary blood flow and coronary vascular resistance (-31±4% versus -7±3% [P<.001] and 53±12% versus 12±8% [P<.02], respectively). Conclusions: The present study demonstrates an attenuated coronary vasoconstrictor response to ET-1 with an enhanced vasoconstrictor response to sarafotoxin and suggests an alteration in coronary ET receptor sensitivity in experimental heart failure.

Original languageEnglish (US)
Pages (from-to)646-651
Number of pages6
JournalCirculation
Volume93
Issue number4
DOIs
StatePublished - Feb 15 1996

Keywords

  • arteries
  • endothelin
  • heart failure
  • receptors
  • vasoconstriction

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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