Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body

Markus Babst, David J. Katzmann, William B. Snyder, Beverly Wendland, Scott D. Emr

Research output: Contribution to journalArticle

469 Scopus citations

Abstract

Sorting of ubiquitinated endosomal membrane proteins into the MVB pathway is executed by the class E Vps protein complexes ESCRT-I, -II, and -III, and the AAA-type ATPase Vps4. This study characterizes ESCRT-II, a soluble ∼155 kDa protein complex formed by the class E Vps proteins Vps22, Vps25, and Vps36. This protein complex transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo. We propose that the ESCRT complexes perform a coordinated cascade of events to select and sort MVB cargoes for delivery to the lumen of the vacuole/lysosome.

Original languageEnglish (US)
Pages (from-to)283-289
Number of pages7
JournalDevelopmental Cell
Volume3
Issue number2
DOIs
StatePublished - Aug 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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