Effects of salbutamol on intracellular calcium oscillations in porcine airway smooth muscle

Y. S. Prakash, H. F.M. Van Der Heijden, M. S. Kannan, G. C. Sieck

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Relaxation of airway smooth muscle (ASM) by β-adrenoceptor agonists involves reduction of intracellular Ca2+ concentration ([Ca2+](i)). In porcine ASM cells, acetylcholine induces [Ca2+](i) oscillations that display frequency modulation by agonist concentration and basal [Ca2+](i). We used real-time confocal microscopy to examine the effect of salbutamol (1 nM to 1 μM), a β2-adrenoceptor agonist, on [Ca2+](i) oscillations in freshly dissociated porcine ASM cells. Salbutamol decreased the frequency of [Ca2+](i) oscillations in a concentration-dependent fashion, completely inhibiting the oscillations at 1μM. These effects were mimicked by a cell- permeant analog of adenosine 3',5'-cyclic monophosphate. The inhibitory effect of salbutamol was partially reversed by BAY K 8644. Salbutamol reduced [Ca2+)(i) even when sarcoplasmic reticulum (SR) Ca2+ reuptake and Ca2+ influx were blocked. Lanthanum blockade of Ca2+ efflux attenuated the inhibitory effect of salbutamol on [Ca2+](i). The [Ca2+](i) response to caffeine was unaffected by salbutamol. On the basis of these results, we conclude that β2-adrenoceptor agonists have little effect on SR Ca2+ release in ASM cells but reduce [ Ca2+](i) by inhibiting Ca2+ influx through voltage-gated channels and by enhancing Ca2+ efflux.

Original languageEnglish (US)
Pages (from-to)1836-1843
Number of pages8
JournalJournal of applied physiology
Volume82
Issue number6
DOIs
StatePublished - Jun 1997

Keywords

  • Asthma
  • Calcium channel
  • Calcium efflux
  • Confocal imaging
  • β-adrenoceptor agonists

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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