Effects of insulin-like growth factor-I on cultured human coronary artery smooth muscle cells

Antoni Bayes-Genis, Robert S. Schwartz, Laurie K. Bale, Cheryl A. Conover

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The growth-promoting effects of insulin-like growth factor-I (IGF-I) appear to be different in vascular smooth muscle cells from various segments of the arterial tree. Little information exists on human coronary artery smooth muscle cells (CoSMC), the primary elements of coronary atherosclerosis and post-angioplasty restenosis. In this study we determined the effects of IGF-I on cultured human CoSMC. Type I IGF receptors (IGF-R) were present on CoSMC as assessed by affinity cross-linking of 125I-IGF-I to monolayer cultures. IGF-I was a weak mitogen, 1.5-fold increase in [3H]thymidine incorporation, for CoSMC. However, IGF-I had a potent motility effect on CoSMC with a 314 ± 12% increase in cell migration (P < 0.001), comparable to that of 5% FBS. IGF-I-stimulated motility was partially inhibited by αIR-3, a specific IGF-R inhibitor (P < 0.05). Addition of kistrin, a disintegrin, or LM609, a specific αvβ3 integrin neutralizing antibody, abolished IGF-I-stimulated migration (P < 0.001). This study indicates that IGF-I is a potent motility agent for human CoSMC via the αvβ3 integrin receptor, but exerts little mitogenic effect. Because CoSMC migration plays a crucial role in atherosclerosis and restenosis, IGF-I blockade has the potential to limit lumen reduction.

Original languageEnglish (US)
Pages (from-to)246-253
Number of pages8
JournalGrowth Hormone and IGF Research
Volume13
Issue number5
DOIs
StatePublished - Oct 2003

Keywords

  • IGF-I
  • Migration
  • Vascular smooth muscle cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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