Effects of hypoxia and nitric oxide on ferritin content of alveolar cells

Jacqueline J. Smith, Amy R. O'Brien-Ladner, Chris R. Kaiser, Lewis J. Wesselius

Research output: Contribution to journalArticle

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Abstract

Concentrations of ferritin in alveolar cells and on the alveolar surface are increased in patients with a variety of respiratory disorders. Ferritin synthesis by cells is modulated by iron content but is also influenced by stimuli other than iron. In this study we sought to determine whether in vitro exposure to hypoxia- or nitric oxide (NO)-induced ferritin accumulation or release by human alveolar macrophages (AMs) or a lung cancer-derived epithelial cell line (A549). Changes in cell content of iron and ferritin (L- and H-types), as well as ferritin content of cell supernatants, were determined after in vitro exposure to hypoxia (1% or 10% O2, 18 hours) or the NO donor S-nitroso-N-acetylpenicillamine (SNAP, 0.01-1.0 mmol/L, 18 hours). Exposure to 1% O2 increased ferritin content in both cell types (>fourfold increase; P < .005) without changing iron content. Treatment with SNAP increased ferritin content of A549 cells in a dose-dependent manner, whereas treatment of AMs decreased cellular iron and ferritin content and increased supernate ferritin content. Pretreatment of cells with N-acetylcysteine (500 μmol/L) reduced hypoxia-induced ferritin accumulation in alveolar cells and completely inhibited NO-induced ferritin accumulation in A549 cells. These findings indicate that exposure to 1% O2can increase ferritin content in alveolar cells, whereas NO can increase ferritin content (A549 cells) or decrease ferritin content (AMs).

Original languageEnglish (US)
Pages (from-to)309-317
Number of pages9
JournalJournal of Laboratory and Clinical Medicine
Volume141
Issue number5
DOIs
StatePublished - May 1 2003

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Alveolar Epithelial Cells
Ferritins
Nitric Oxide
Iron
Alveolar Macrophages
Apoferritins
Hypoxia
S-Nitroso-N-Acetylpenicillamine
Nitric Oxide Donors
Acetylcysteine

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

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Effects of hypoxia and nitric oxide on ferritin content of alveolar cells. / Smith, Jacqueline J.; O'Brien-Ladner, Amy R.; Kaiser, Chris R.; Wesselius, Lewis J.

In: Journal of Laboratory and Clinical Medicine, Vol. 141, No. 5, 01.05.2003, p. 309-317.

Research output: Contribution to journalArticle

Smith, Jacqueline J. ; O'Brien-Ladner, Amy R. ; Kaiser, Chris R. ; Wesselius, Lewis J. / Effects of hypoxia and nitric oxide on ferritin content of alveolar cells. In: Journal of Laboratory and Clinical Medicine. 2003 ; Vol. 141, No. 5. pp. 309-317.
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