Effects of 5-fluorouracil and leucovorin in spheroids: A model for solid tumours

C. Erlichman, A. Wu

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We determined the cytotoxity of 5FU alone or with leucovorin in MGH-U1 cells growing as monolayers or spheroids. The 5FU IC50 in spheroid and monolayers was 100 μM in both culture systems. The IC50 of 5FU and 5FU and 100 μM leucovorin in spheroids and monolayers was 100 μM and 50 μM, respectively. [H3]5FU reached cells at all depths of the spheroid viable rim. Cells isolated from increasing depths of the spheroid viable rim demonstrated increasing resistance to 5FU. 5FU cytotoxicity is increased by leucovorin in monolayer but not spheroids, penetration is not limiting in 5FU cytotoxicity in spheroids and there is increasing resistance to 5FU in the viable rim.

Original languageEnglish (US)
Pages (from-to)671-675
Number of pages5
JournalAnticancer research
Volume11
Issue number2
StatePublished - Jan 1 1991

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Keywords

  • 5-fluorouracil
  • leucovorin
  • spheroids

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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