Abstract
The present stud\ was designed to determine whether expression of recombinant endothelial nitric oxide synthase (eNOS) may affect vasomotor iunaion of canine cerebral arteries- Replication-defective adenoviral vectors encoding hovine eNOS (AdCMVNOS) gene and bacterial -galactosidasc (AdCMVLau/) gene were constructed. Kx vivo gene transfer was achieved by 30 mm incubation ofbasibr artery rings in minimal essential medium {37°C) increasing.! liters ) 10'. and 3.5 x 101" pfu/ml) of adenoviral vectors. Foru -eight hours following gene transier arteries were examined for lunuionai expression of recombinant gene, using histochemical staining, iMiinetric tension recordings and c\eiic GMP radioimmunoassay. Transfection u ith -\dC MVl.acX or AdCMVNOS resulted in a viral tiler-dependent increase MI reconibinant protein expression m adventitia. In arteries transfected with Ad< MVNOS (but not AdCMVl ac/) contractile responses to uridine 5'inph<>sphate 110 -]0 'Ml were significantly reduced. ['Expression of K.-c.mibtnant -Vdt'MX'NOS resulted in increased basal production of cyclic ( 'MP that vvu-, inhibited by Nl'-monnmethyl-l-arginine. These results suggest !!u': ai successful expression oi'recoinbinant AdCMVNOS can be achieved in iCiebral arteries, and b) eNOS gene transfer may provide a novel approach to icsuire impaired niini- oxide production in diseased blood vessels.
| Original language | English (US) |
|---|---|
| Pages (from-to) | A303 |
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 3 |
| State | Published - 1996 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Genetics