Effect of mechanical stimulation on bone marrow stromal cell-seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair

Ting Wu Qin, Yu Long Sun, Andrew R. Thoreson, Scott P. Steinmann, Peter C Amadio, Kai Nan An, Chunfeng D Zhao

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Cell-based tissue engineered tendons have potential to improve clinical outcomes following rotator cuff repair, especially in large or massive rotator cuff tears, which pose a great clinical challenge. The aim of this study was to develop a method of constructing a functional engineered tendon patch for rotator cuff repair with cyclic mechanical stimulation. Decellularized tendon slices (DTSs) were seeded with BMSCs and subjected to cyclic stretching for 1, 3, or 7 days. The mechanical properties, morphologic characteristics and tendon-related gene expression of the constructs were investigated. Viable BMSCs were observed on the DTS after 7 days. BMSCs penetrated into the DTSs and formed dense cell sheets after 7 days of mechanical stretching. Gene expression of type I collagen, decorin, and tenomodulin significantly increased in cyclically stretched BMSC-DTS constructs compared with the unstrained control group (P<0.05). The ultimate tensile strength and stiffness of the cyclically stretched tendon constructs were similar to the unstrained control group (P>0.05). In conclusion, mechanical stimulation of BMSC-DTS constructs upregulated expression of tendon-related proteins, promoted cell tenogenic differentiation, facilitated cell infiltration and formation of cell sheets, and retained mechanical properties. The patch could be used as a graft to enhance the surgical repair of rotator cuff tears.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalBiomaterials
Volume51
DOIs
StatePublished - May 1 2015

Fingerprint

Rotator Cuff
Tendons
Mesenchymal Stromal Cells
Bone
Repair
Gene expression
Stretching
Decorin
Gene Expression
Mechanical properties
Collagen Type I
Infiltration
Collagen
Grafts
Cell Differentiation
Cells
Tissue
Proteins
Transplants
Control Groups

Keywords

  • Bone marrow stromal cells
  • Stretch
  • Tendon slice
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Effect of mechanical stimulation on bone marrow stromal cell-seeded tendon slice constructs : A potential engineered tendon patch for rotator cuff repair. / Qin, Ting Wu; Sun, Yu Long; Thoreson, Andrew R.; Steinmann, Scott P.; Amadio, Peter C; An, Kai Nan; Zhao, Chunfeng D.

In: Biomaterials, Vol. 51, 01.05.2015, p. 43-50.

Research output: Contribution to journalArticle

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