Effect of endogenous hydrogen sulfide on the transwall gradient of the mouse colon circular smooth muscle

L. Sha, D. R. Linden, G. Farrugia, J. H. Szurszewski

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A transwall gradient in resting membrane potential (RMP) exists across the circular muscle layer in the mouse colon. This gradient is dependent on endogenous generation of CO. H2S is also generated in muscle layers of the mouse colon. The effect of endogenously generated H2S on the transwall gradient is not known. The aim was to investigate the role of endogenous H2S. Our results showed that the CSE inhibitor dl-propargylglycine (PAG, 500 μm) had no effect on the transwall gradient. However, in preparations pretreated with the nitric oxide synthase inhibitor N-nitro-l-arginine (l-NNA, 200 μm) and in nNOS-knockout (KO) mouse preparations, PAG shifted the transwall gradient in the depolarizing direction. In CSE-KO-nNOS-KO mice, the gradient was shifted in the depolarizing direction. Endogenous generation of NO was significantly higher in muscle preparations of CSE-KO mice compared to wild-type (WT) mice. The amplitude of NO-mediated slow inhibitory junction potentials (S-IJPs) evoked by electric field stimulation was significantly higher in CSE-KO mouse preparations compared to the amplitude of S-IJPs in wild-type mouse preparations. CSE was present in all submucosal ganglion neurons and in almost all myenteric ganglion neurons. Eleven per cent of CSE positive neurons in the submucosal plexus and 50% of CSE positive neurons in the myenteric plexus also contained nNOS. Our results suggest that endogenously generated H2S acts as a stealth hyperpolarizing factor on smooth muscle cells to maintain the CO-dependent transwall gradient and inhibits NO production from nNOS.

Original languageEnglish (US)
Pages (from-to)1077-1089
Number of pages13
JournalJournal of Physiology
Volume592
Issue number5
DOIs
StatePublished - Mar 1 2014

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Effect of endogenous hydrogen sulfide on the transwall gradient of the mouse colon circular smooth muscle'. Together they form a unique fingerprint.

Cite this