TY - JOUR
T1 - Effect of arginine vasopressin on the canine epicardial coronary artery. Experiments on V1-receptor-mediated production of nitric oxide
AU - Evora, Paulo Roberto B.
AU - Pearson, Paul J.
AU - Rodrigues, Alfredo J.
AU - Viaro, Fernanda
AU - Schaff, Hartzell V.
PY - 2003/4
Y1 - 2003/4
N2 - Objective -To determine whether arginine vasopressin releases endothelium-derived nitric oxide (EDNO) from the epicardial coronary artery. Methods - We studied segments of canine left circumflex coronary arteries suspended in organ chambers to measure isometric force. The coronary artery segments were contracted with prostaglandin F2α (2 × 10-6 M) and exposed to a unique, strong arginine vasopressin concentration (10-6 M) or titrated concentrations (10-9 a 10-5 M). Results - The unique dose of arginine vasopressin concentration (10-6 M) induced transient, but significant (p<0.05), relaxation in arterial segments with endothelium, and an increase, not significant, in tension in arteries without endothelium. Endothelium-dependent relaxation to arginine vasopressin was inhibited by Ng-monomethyl-L-arginine (L-NMMA, 10-5 M) or NG-nitro-L- arginine (L-NOARG) (10-4 M), 2 inhibitors of nitric oxide synthesis from L-arginine. Exogenous L-arginine (10-4 M), but not D-arginine (10-4 M), reversed the inhibitory effect of L-NMMA on vasopressin-mediated vasorelaxation. Endothelium dependent relaxation to vasopressin was also reversibly inhibited by the vasopressin V 1-receptor blocker d(CH2)5Try(Me) arginine vasopressin (10-6 M) (n=6, P<0.05). Conclusion - Vasopressin acts through V1 endothelial receptors to stimulate nitric oxide release from L-arginine.
AB - Objective -To determine whether arginine vasopressin releases endothelium-derived nitric oxide (EDNO) from the epicardial coronary artery. Methods - We studied segments of canine left circumflex coronary arteries suspended in organ chambers to measure isometric force. The coronary artery segments were contracted with prostaglandin F2α (2 × 10-6 M) and exposed to a unique, strong arginine vasopressin concentration (10-6 M) or titrated concentrations (10-9 a 10-5 M). Results - The unique dose of arginine vasopressin concentration (10-6 M) induced transient, but significant (p<0.05), relaxation in arterial segments with endothelium, and an increase, not significant, in tension in arteries without endothelium. Endothelium-dependent relaxation to arginine vasopressin was inhibited by Ng-monomethyl-L-arginine (L-NMMA, 10-5 M) or NG-nitro-L- arginine (L-NOARG) (10-4 M), 2 inhibitors of nitric oxide synthesis from L-arginine. Exogenous L-arginine (10-4 M), but not D-arginine (10-4 M), reversed the inhibitory effect of L-NMMA on vasopressin-mediated vasorelaxation. Endothelium dependent relaxation to vasopressin was also reversibly inhibited by the vasopressin V 1-receptor blocker d(CH2)5Try(Me) arginine vasopressin (10-6 M) (n=6, P<0.05). Conclusion - Vasopressin acts through V1 endothelial receptors to stimulate nitric oxide release from L-arginine.
KW - Coronary artery
KW - Endothelium
KW - Nitric oxide
KW - Vasopressin
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U2 - 10.1590/S0066-782X2003000500002
DO - 10.1590/S0066-782X2003000500002
M3 - Article
C2 - 12792714
AN - SCOPUS:3343007804
SN - 0066-782X
VL - 80
SP - 489
EP - 494
JO - Arquivos brasileiros de cardiologia
JF - Arquivos brasileiros de cardiologia
IS - 5
ER -