Effect of arginine vasopressin on the canine epicardial coronary artery. Experiments on V1-receptor-mediated production of nitric oxide

Paulo Roberto B. Evora, Paul J. Pearson, Alfredo J. Rodrigues, Fernanda Viaro, Hartzell V. Schaff

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Objective -To determine whether arginine vasopressin releases endothelium-derived nitric oxide (EDNO) from the epicardial coronary artery. Methods - We studied segments of canine left circumflex coronary arteries suspended in organ chambers to measure isometric force. The coronary artery segments were contracted with prostaglandin F (2 × 10-6 M) and exposed to a unique, strong arginine vasopressin concentration (10-6 M) or titrated concentrations (10-9 a 10-5 M). Results - The unique dose of arginine vasopressin concentration (10-6 M) induced transient, but significant (p<0.05), relaxation in arterial segments with endothelium, and an increase, not significant, in tension in arteries without endothelium. Endothelium-dependent relaxation to arginine vasopressin was inhibited by Ng-monomethyl-L-arginine (L-NMMA, 10-5 M) or NG-nitro-L- arginine (L-NOARG) (10-4 M), 2 inhibitors of nitric oxide synthesis from L-arginine. Exogenous L-arginine (10-4 M), but not D-arginine (10-4 M), reversed the inhibitory effect of L-NMMA on vasopressin-mediated vasorelaxation. Endothelium dependent relaxation to vasopressin was also reversibly inhibited by the vasopressin V 1-receptor blocker d(CH2)5Try(Me) arginine vasopressin (10-6 M) (n=6, P<0.05). Conclusion - Vasopressin acts through V1 endothelial receptors to stimulate nitric oxide release from L-arginine.

Original languageEnglish (US)
Pages (from-to)489-494
Number of pages6
JournalArquivos brasileiros de cardiologia
Volume80
Issue number5
DOIs
StatePublished - Apr 2003

Keywords

  • Coronary artery
  • Endothelium
  • Nitric oxide
  • Vasopressin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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