Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5 and Homer

Noah W. Gray, Lawrence Fourgeaud, Bing Huang, Jing Chen, Hong Cao, Barbara J. Oswald, Agnès Hémar, Mark A Mc Niven

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The dynamins comprise a large family of mechanoenzymes known to participate in membrane modeling events [1, 2]. All three conventional dynamin genes (Dyn1, Dyn2, Dyn3) are expressed in mammalian brain and produce more than 27 different dynamin proteins as a result of alternative splicing [3]. Past studies have suggested that Dyn1 participates in specialized neuronal functions such as rapid synaptic vesicle recycling [4], while Dyn2 may mediate the conventional clathrin-mediated uptake of surface receptors [5]. Currently, the distribution, expression, and function of Dyn3 in neurons, or in any other cell type, are completely undefined. Here, we demonstrate that Dyn1 and Dyn3 localize differentially in the synapse. Dyn1 concentrates within the presynaptic compartment, while Dyn3 localizes to dendritic spine tips. Within the postsynaptic density (PSD), we found Dyn3, but not Dyn1, to be part of a biochemically isolated complex comprised of Homer and metabotropic glutamate receptors. Finally, although dominant-negative Dyn3 did not seem to inhibit receptor endocytosis, overexpression of a specific Dyn3 spliced variant in mature neurons caused a marked remodeling of dendritic spines. These data suggest that Dyn3 is a postsynaptic dynamin and, like its binding partner Homer, plays a significant role in dendritic spine morphogenesis and remodeling.

Original languageEnglish (US)
Pages (from-to)510-515
Number of pages6
JournalCurrent Biology
Volume13
Issue number6
DOIs
StatePublished - Mar 18 2003

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Dynamin III
Dynamins
Dendritic Spines
Neurons
neurons
Post-Synaptic Density
clathrin
Clathrin
receptors
Metabotropic Glutamate Receptors
Neuronal Plasticity
Synaptic Vesicles
alternative splicing
Alternative Splicing
endocytosis
synapse
Endocytosis
Morphogenesis
Synapses
recycling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5 and Homer. / Gray, Noah W.; Fourgeaud, Lawrence; Huang, Bing; Chen, Jing; Cao, Hong; Oswald, Barbara J.; Hémar, Agnès; Mc Niven, Mark A.

In: Current Biology, Vol. 13, No. 6, 18.03.2003, p. 510-515.

Research output: Contribution to journalArticle

Gray, NW, Fourgeaud, L, Huang, B, Chen, J, Cao, H, Oswald, BJ, Hémar, A & Mc Niven, MA 2003, 'Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5 and Homer', Current Biology, vol. 13, no. 6, pp. 510-515. https://doi.org/10.1016/S0960-9822(03)00136-2
Gray, Noah W. ; Fourgeaud, Lawrence ; Huang, Bing ; Chen, Jing ; Cao, Hong ; Oswald, Barbara J. ; Hémar, Agnès ; Mc Niven, Mark A. / Dynamin 3 is a component of the postsynapse, where it interacts with mGluR5 and Homer. In: Current Biology. 2003 ; Vol. 13, No. 6. pp. 510-515.
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