Duodenoesophageal reflux and the development of esophageal adenocarcinoma in rats

S. E A Attwood, Thomas Christopher Smyrk, T. R. DeMeester, S. S. Mirvish, H. J. Stein, R. A. Hinder

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

Background. The carcinogenic effect of duodenoesophageal reflux, gastroesophageal reflux, and nitrosamines was studied in the rat esophagus. Methods. Twenty male Sprague-Dawley rats underwent esophagogastroplasty to produce gastroesophageal reflux and 60 underwent duodenoesophageal anastomosis to produce duodenoesophageal reflux. Forty-three animals underwent no operation and acted as controls. Carcinogens known to produce squamous tumors in the rat esophagus (2,6-dimethylnitrosomorpholine [DMNM] or methyl-n-amylnitrosamine [MNAN]) were tested in each group. Results. The rate of squamous carcinoma was 25% for rats with DMNM alone, 30% for rats with MNAN alone, and 20% for rats with induced gastroesophageal reflux plus DMNM. The rate of malignant change rose to 80% in rats with induced duodenoesophageal reflux and DMNM and 67% with duodenoesophageal reflux and MNAN. With duodenoesophageal reflux, 50% of tumors were adenocarcinoma, in contrast to 100% squamous differentiation of tumors in rats given the carcinogens with esophagogastroplasty or no operation. Conclusion. The presence of duodenoesophageal reflux increased the frequency and changed the histologic type of esophageal cancer in nitrosamine-treated rats. This indicates that duodenoesophageal reflux plays a role in the development of esophageal adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)503-510
Number of pages8
JournalSurgery
Volume111
Issue number5
StatePublished - 1992
Externally publishedYes

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Gastroesophageal Reflux
N-amyl-N-methylnitrosamine
Adenocarcinoma
Nitrosamines
Carcinogens
Esophagus
Neoplasms
Esophageal Neoplasms
Sprague Dawley Rats
Squamous Cell Carcinoma
N-nitroso-2,6-dimethylmorpholine

ASJC Scopus subject areas

  • Surgery

Cite this

Attwood, S. E. A., Smyrk, T. C., DeMeester, T. R., Mirvish, S. S., Stein, H. J., & Hinder, R. A. (1992). Duodenoesophageal reflux and the development of esophageal adenocarcinoma in rats. Surgery, 111(5), 503-510.

Duodenoesophageal reflux and the development of esophageal adenocarcinoma in rats. / Attwood, S. E A; Smyrk, Thomas Christopher; DeMeester, T. R.; Mirvish, S. S.; Stein, H. J.; Hinder, R. A.

In: Surgery, Vol. 111, No. 5, 1992, p. 503-510.

Research output: Contribution to journalArticle

Attwood, SEA, Smyrk, TC, DeMeester, TR, Mirvish, SS, Stein, HJ & Hinder, RA 1992, 'Duodenoesophageal reflux and the development of esophageal adenocarcinoma in rats', Surgery, vol. 111, no. 5, pp. 503-510.
Attwood SEA, Smyrk TC, DeMeester TR, Mirvish SS, Stein HJ, Hinder RA. Duodenoesophageal reflux and the development of esophageal adenocarcinoma in rats. Surgery. 1992;111(5):503-510.
Attwood, S. E A ; Smyrk, Thomas Christopher ; DeMeester, T. R. ; Mirvish, S. S. ; Stein, H. J. ; Hinder, R. A. / Duodenoesophageal reflux and the development of esophageal adenocarcinoma in rats. In: Surgery. 1992 ; Vol. 111, No. 5. pp. 503-510.
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AB - Background. The carcinogenic effect of duodenoesophageal reflux, gastroesophageal reflux, and nitrosamines was studied in the rat esophagus. Methods. Twenty male Sprague-Dawley rats underwent esophagogastroplasty to produce gastroesophageal reflux and 60 underwent duodenoesophageal anastomosis to produce duodenoesophageal reflux. Forty-three animals underwent no operation and acted as controls. Carcinogens known to produce squamous tumors in the rat esophagus (2,6-dimethylnitrosomorpholine [DMNM] or methyl-n-amylnitrosamine [MNAN]) were tested in each group. Results. The rate of squamous carcinoma was 25% for rats with DMNM alone, 30% for rats with MNAN alone, and 20% for rats with induced gastroesophageal reflux plus DMNM. The rate of malignant change rose to 80% in rats with induced duodenoesophageal reflux and DMNM and 67% with duodenoesophageal reflux and MNAN. With duodenoesophageal reflux, 50% of tumors were adenocarcinoma, in contrast to 100% squamous differentiation of tumors in rats given the carcinogens with esophagogastroplasty or no operation. Conclusion. The presence of duodenoesophageal reflux increased the frequency and changed the histologic type of esophageal cancer in nitrosamine-treated rats. This indicates that duodenoesophageal reflux plays a role in the development of esophageal adenocarcinoma.

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