Duodenal mastocytosis, eosinophilia and intraepithelial lymphocytosis as possible disease markers in the irritable bowel syndrome and functional dyspepsia

M. M. Walker, N. J. Talley, M. Prabhakar, C. J. Pennaneac'H, P. Aro, J. Ronkainen, T. Storskrubb, W. S. Harmsen, A. R. Zinsmeister, L. Agreus

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Background Irritable bowel syndrome (IBS) and functional dyspepsia (FD) are common functional disorders without defined pathology. Mast cells and eosinophils interact with T lymphocytes and may alter enteric nerve and smooth muscle function. Aim To examine mast cell, eosinophil and intraepithelial lymphocyte populations in duodenal biopsies of subjects with IBS and FD. Methods A random sample of an adult Swedish population (n = 1001; mean age 54 years; 51% female) underwent upper endoscopy and biopsy; 51 cases with FD and 41 cases with IBS were compared with 48 randomly selected controls. Eosinophils were identified by light microscopy; mast cells by immunocytochemistry (CD117). Intraepithelial lymphocytes were counted per 100 enterocytes. Cell counts were quantified by counting the number per high power field (HPF) in 5HPFs in the bulb (D1) and second part of duodenum (D2), summed over 5HPFs at each site. Results Cases and controls showed similar demographics. Compared to controls, IELs in IBS-constipation were significantly increased (P = 0.005). Mast cells were significantly increased in IBS in D2 (P < 0.001), while eosinophils were significantly increased in FD in D1 and D2 (P < 0.001). Conclusion Duodenal mast cell hyperplasia is linked to IBS and eosinophilia to FD, and duodenal biopsy may identify subsets of these disorders.

Original languageEnglish (US)
Pages (from-to)765-773
Number of pages9
JournalAlimentary Pharmacology and Therapeutics
Volume29
Issue number7
DOIs
StatePublished - Apr 2009

Fingerprint

Mastocytosis
Lymphocytosis
Irritable Bowel Syndrome
Dyspepsia
Eosinophilia
Mast Cells
Eosinophils
Biopsy
Lymphocytes
Enterocytes
Constipation
Duodenum
Population
Endoscopy
Hyperplasia
Smooth Muscle
Microscopy
Cell Count
Immunohistochemistry
Demography

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Duodenal mastocytosis, eosinophilia and intraepithelial lymphocytosis as possible disease markers in the irritable bowel syndrome and functional dyspepsia. / Walker, M. M.; Talley, N. J.; Prabhakar, M.; Pennaneac'H, C. J.; Aro, P.; Ronkainen, J.; Storskrubb, T.; Harmsen, W. S.; Zinsmeister, A. R.; Agreus, L.

In: Alimentary Pharmacology and Therapeutics, Vol. 29, No. 7, 04.2009, p. 765-773.

Research output: Contribution to journalArticle

Walker, MM, Talley, NJ, Prabhakar, M, Pennaneac'H, CJ, Aro, P, Ronkainen, J, Storskrubb, T, Harmsen, WS, Zinsmeister, AR & Agreus, L 2009, 'Duodenal mastocytosis, eosinophilia and intraepithelial lymphocytosis as possible disease markers in the irritable bowel syndrome and functional dyspepsia', Alimentary Pharmacology and Therapeutics, vol. 29, no. 7, pp. 765-773. https://doi.org/10.1111/j.1365-2036.2009.03937.x
Walker, M. M. ; Talley, N. J. ; Prabhakar, M. ; Pennaneac'H, C. J. ; Aro, P. ; Ronkainen, J. ; Storskrubb, T. ; Harmsen, W. S. ; Zinsmeister, A. R. ; Agreus, L. / Duodenal mastocytosis, eosinophilia and intraepithelial lymphocytosis as possible disease markers in the irritable bowel syndrome and functional dyspepsia. In: Alimentary Pharmacology and Therapeutics. 2009 ; Vol. 29, No. 7. pp. 765-773.
@article{1fe2dab7e1eb4a758b4e24f545ead1a5,
title = "Duodenal mastocytosis, eosinophilia and intraepithelial lymphocytosis as possible disease markers in the irritable bowel syndrome and functional dyspepsia",
abstract = "Background Irritable bowel syndrome (IBS) and functional dyspepsia (FD) are common functional disorders without defined pathology. Mast cells and eosinophils interact with T lymphocytes and may alter enteric nerve and smooth muscle function. Aim To examine mast cell, eosinophil and intraepithelial lymphocyte populations in duodenal biopsies of subjects with IBS and FD. Methods A random sample of an adult Swedish population (n = 1001; mean age 54 years; 51{\%} female) underwent upper endoscopy and biopsy; 51 cases with FD and 41 cases with IBS were compared with 48 randomly selected controls. Eosinophils were identified by light microscopy; mast cells by immunocytochemistry (CD117). Intraepithelial lymphocytes were counted per 100 enterocytes. Cell counts were quantified by counting the number per high power field (HPF) in 5HPFs in the bulb (D1) and second part of duodenum (D2), summed over 5HPFs at each site. Results Cases and controls showed similar demographics. Compared to controls, IELs in IBS-constipation were significantly increased (P = 0.005). Mast cells were significantly increased in IBS in D2 (P < 0.001), while eosinophils were significantly increased in FD in D1 and D2 (P < 0.001). Conclusion Duodenal mast cell hyperplasia is linked to IBS and eosinophilia to FD, and duodenal biopsy may identify subsets of these disorders.",
author = "Walker, {M. M.} and Talley, {N. J.} and M. Prabhakar and Pennaneac'H, {C. J.} and P. Aro and J. Ronkainen and T. Storskrubb and Harmsen, {W. S.} and Zinsmeister, {A. R.} and L. Agreus",
year = "2009",
month = "4",
doi = "10.1111/j.1365-2036.2009.03937.x",
language = "English (US)",
volume = "29",
pages = "765--773",
journal = "Alimentary Pharmacology and Therapeutics",
issn = "0269-2813",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Duodenal mastocytosis, eosinophilia and intraepithelial lymphocytosis as possible disease markers in the irritable bowel syndrome and functional dyspepsia

AU - Walker, M. M.

AU - Talley, N. J.

AU - Prabhakar, M.

AU - Pennaneac'H, C. J.

AU - Aro, P.

AU - Ronkainen, J.

AU - Storskrubb, T.

AU - Harmsen, W. S.

AU - Zinsmeister, A. R.

AU - Agreus, L.

PY - 2009/4

Y1 - 2009/4

N2 - Background Irritable bowel syndrome (IBS) and functional dyspepsia (FD) are common functional disorders without defined pathology. Mast cells and eosinophils interact with T lymphocytes and may alter enteric nerve and smooth muscle function. Aim To examine mast cell, eosinophil and intraepithelial lymphocyte populations in duodenal biopsies of subjects with IBS and FD. Methods A random sample of an adult Swedish population (n = 1001; mean age 54 years; 51% female) underwent upper endoscopy and biopsy; 51 cases with FD and 41 cases with IBS were compared with 48 randomly selected controls. Eosinophils were identified by light microscopy; mast cells by immunocytochemistry (CD117). Intraepithelial lymphocytes were counted per 100 enterocytes. Cell counts were quantified by counting the number per high power field (HPF) in 5HPFs in the bulb (D1) and second part of duodenum (D2), summed over 5HPFs at each site. Results Cases and controls showed similar demographics. Compared to controls, IELs in IBS-constipation were significantly increased (P = 0.005). Mast cells were significantly increased in IBS in D2 (P < 0.001), while eosinophils were significantly increased in FD in D1 and D2 (P < 0.001). Conclusion Duodenal mast cell hyperplasia is linked to IBS and eosinophilia to FD, and duodenal biopsy may identify subsets of these disorders.

AB - Background Irritable bowel syndrome (IBS) and functional dyspepsia (FD) are common functional disorders without defined pathology. Mast cells and eosinophils interact with T lymphocytes and may alter enteric nerve and smooth muscle function. Aim To examine mast cell, eosinophil and intraepithelial lymphocyte populations in duodenal biopsies of subjects with IBS and FD. Methods A random sample of an adult Swedish population (n = 1001; mean age 54 years; 51% female) underwent upper endoscopy and biopsy; 51 cases with FD and 41 cases with IBS were compared with 48 randomly selected controls. Eosinophils were identified by light microscopy; mast cells by immunocytochemistry (CD117). Intraepithelial lymphocytes were counted per 100 enterocytes. Cell counts were quantified by counting the number per high power field (HPF) in 5HPFs in the bulb (D1) and second part of duodenum (D2), summed over 5HPFs at each site. Results Cases and controls showed similar demographics. Compared to controls, IELs in IBS-constipation were significantly increased (P = 0.005). Mast cells were significantly increased in IBS in D2 (P < 0.001), while eosinophils were significantly increased in FD in D1 and D2 (P < 0.001). Conclusion Duodenal mast cell hyperplasia is linked to IBS and eosinophilia to FD, and duodenal biopsy may identify subsets of these disorders.

UR - http://www.scopus.com/inward/record.url?scp=61849182973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61849182973&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2036.2009.03937.x

DO - 10.1111/j.1365-2036.2009.03937.x

M3 - Article

C2 - 19183150

AN - SCOPUS:61849182973

VL - 29

SP - 765

EP - 773

JO - Alimentary Pharmacology and Therapeutics

JF - Alimentary Pharmacology and Therapeutics

SN - 0269-2813

IS - 7

ER -