TY - JOUR
T1 - Dose-dependent effect of EGF on migration and differentiation of adult subventricular zone astrocytes
AU - Gonzalez-Perez, Oscar
AU - Quiñones-Hinojosa, Alfredo
PY - 2010/6
Y1 - 2010/6
N2 - Adult neural stem cells (NSCs) are located in the subventricular zone (SVZ), a specialized brain niche located on the walls of the lateral ventricle. Under physiological conditions, NSCs generate a large number of young neurons and some oligodendrocytes, however the mechanisms controlling cell proliferation and migration are unclear. In vitro, epidermal growth factor (EGF) signaling has been shown to be an important mediator of cell proliferation and migration in the adult brain; however, the primary SVZ progenitors that respond to EGF are not well known. In this study, we isolated SVZ type-B astrocytes and cultured them under different EGF concentrations. We found a dose-dependent effect of EGF on proliferation rates and migration of SVZ type-B astrocytes. We found that GFAP+ type-B astrocytes gave rise to highly migratory and proliferating cells that expressed Olig2 and NG2. After EGF withdrawal, a signifi-cant number of EGF-stimulated cells differentiated into S100β+/O41 oligodendrocytes. This study provides new insights about the production of oligodendrocytes derived from the astrocyte NSCs residing in the adult SVZ. To be able to manipulate the endogenous adult progenitors, it is crucial to identify and isolate the responding primary precursors and determine the extracellular signals that regulate their cell division, migration, and fate.
AB - Adult neural stem cells (NSCs) are located in the subventricular zone (SVZ), a specialized brain niche located on the walls of the lateral ventricle. Under physiological conditions, NSCs generate a large number of young neurons and some oligodendrocytes, however the mechanisms controlling cell proliferation and migration are unclear. In vitro, epidermal growth factor (EGF) signaling has been shown to be an important mediator of cell proliferation and migration in the adult brain; however, the primary SVZ progenitors that respond to EGF are not well known. In this study, we isolated SVZ type-B astrocytes and cultured them under different EGF concentrations. We found a dose-dependent effect of EGF on proliferation rates and migration of SVZ type-B astrocytes. We found that GFAP+ type-B astrocytes gave rise to highly migratory and proliferating cells that expressed Olig2 and NG2. After EGF withdrawal, a signifi-cant number of EGF-stimulated cells differentiated into S100β+/O41 oligodendrocytes. This study provides new insights about the production of oligodendrocytes derived from the astrocyte NSCs residing in the adult SVZ. To be able to manipulate the endogenous adult progenitors, it is crucial to identify and isolate the responding primary precursors and determine the extracellular signals that regulate their cell division, migration, and fate.
KW - Neural stem cells
KW - Oligodendrocytes
KW - Subventricular zone
UR - http://www.scopus.com/inward/record.url?scp=77952578317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952578317&partnerID=8YFLogxK
U2 - 10.1002/glia.20979
DO - 10.1002/glia.20979
M3 - Article
C2 - 20187143
AN - SCOPUS:77952578317
SN - 0894-1491
VL - 58
SP - 975
EP - 983
JO - Glia
JF - Glia
IS - 8
ER -