DNA-templated organic synthesis and selection of a library of macrocycles

Zev J. Gartner, Brian N. Tse, Rozalina Grubina, Jeffrey B. Doyon, Thomas M. Snyder, David R. Liu

Research output: Contribution to journalArticlepeer-review

328 Scopus citations

Abstract

The translation of nucleic acid libraries into corresponding synthetic compounds would enable selection and amplification principles to be applied to man-made molecules. We used multistep DNA-templated organic synthesis to translate libraries of DNA sequences, each containing three "codons," into libraries of sequence-programmed synthetic small-molecule macrocycles. The resulting DNA-macrocycle conjugates were subjected to in vitro selections for protein affinity. The identity of a single macrocycle possessing known target protein affinity was inferred through the sequence of the amplified DNA template surviving the selection. This work represents the translation, selection, and amplification of libraries of nucleic acids encoding synthetic small molecules rather than biological macromolecules.

Original languageEnglish (US)
Pages (from-to)1601-1605
Number of pages5
JournalScience
Volume305
Issue number5690
DOIs
StatePublished - Sep 10 2004

ASJC Scopus subject areas

  • General

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