DNA sequence of a human Sm autoimmune antigen. The multigene family contains a processed pseudogene

D. R. Stanford, A. Rohleder, K. Neiswanger, Eric D Wieben

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The sequence of a complementary DNA clone coding for a human autoimmune antigen has been determined. This DNA sequence predicts the amino acid sequence of a small protein ('E') which is associated with small nuclear RNA in human cells. Analysis of the predicted protein sequence suggests that the E protein is not closely related to other nucleic acid binding proteins. Screening of a human genomic DNA library has led to the isolation of several members of the E protein multigene family. Sequence analysis of one member of this family reveals that it is flanked by direct repeats and contains several mutations. One of these mutations, an insertion, terminates the long open reading frame. These features are compatible with the designation of this sequence as a processed pseudogene.

Original languageEnglish (US)
Pages (from-to)9931-9934
Number of pages4
JournalJournal of Biological Chemistry
Volume262
Issue number21
StatePublished - 1987

Fingerprint

Pseudogenes
DNA sequences
Multigene Family
Antigens
Small Nuclear RNA
Proteins
Genomic Library
Nucleic Acid Repetitive Sequences
Insertional Mutagenesis
Protein Sequence Analysis
Gene Library
Nucleic Acids
Open Reading Frames
Sequence Analysis
Amino Acid Sequence
Carrier Proteins
Complementary DNA
Clone Cells
Screening
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

DNA sequence of a human Sm autoimmune antigen. The multigene family contains a processed pseudogene. / Stanford, D. R.; Rohleder, A.; Neiswanger, K.; Wieben, Eric D.

In: Journal of Biological Chemistry, Vol. 262, No. 21, 1987, p. 9931-9934.

Research output: Contribution to journalArticle

Stanford, D. R. ; Rohleder, A. ; Neiswanger, K. ; Wieben, Eric D. / DNA sequence of a human Sm autoimmune antigen. The multigene family contains a processed pseudogene. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 21. pp. 9931-9934.
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