DNA ploidy changes in rhino mouse skin induced by all-trans retinoic acid and retinol

Salvador Gonzalez, Maria Victoria Alcaraz, Florentina Daz, Thomas J Flotte, Ignacio Ferez De Vargas, R. Rox Anderson, Kollias Nik

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: In order to assess the proliferative changes induced by all-trans retinoic acid (PA) and retinol (ROL), we have carried out a study of the DNA content of basal and suprabasal keratinocytes after epicutaneous application on the rhino mouse. Study Design: Skin sections were analyzed stereologically and cytophotometrically using the Feulgen technique. The diploid DNA value (2C) was obtained from hepatocyte nuclei of control animals. Whereas cells in phase G0-G1 will show a 2C content, cells during phase S and in phase G2-M will show DNA values ranging from 2C to 4C and 4C, respectively. Results: Although epidermal thickness (ET) increased significantly in all treated animals, surface density only increased in animals treated with all-trans RA. Quantification of DNA content of basal keratinocytes showed reduction of 2C and 2C-4C populations with a commensurate increase in proportions of cells with 4C and > 4C in the animals treated with 0.025% all-trans RA and ROL. Suprabasal keratinocytes of mice treated with 0.025% all-trans showed a decrease of the 2C population and an increased proportion of cells with 4C. Whereas 0.025% all-trans RA induced an increase of both basal and suprabasal DNA indices, ROL enhanced only the basal DNA index significantly. Conclusion: Animals treated with 0.025% ROL showed a significant increase in the basal proliferative index (PI) while the suprabasal PI remained constant; treatment with 0.025% all-trans RA produced a significant increase of both basal and suprabasal PIs and parakeratotic hyperkeratosis probably due to incomplete differentiation.

Original languageEnglish (US)
Pages (from-to)135-143
Number of pages9
JournalSkin Pharmacology
Volume10
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Ploidies
Tretinoin
Vitamin A
Skin
DNA
Keratinocytes
Cell Cycle Resting Phase
G2 Phase
G1 Phase
Population Dynamics
Diploidy
S Phase
Cell Division
Hepatocytes
Population

Keywords

  • All-trans retinoic acid
  • DNA content
  • Epidermal cell kinetics
  • Retinol
  • Rhino mouse

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Dermatology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Gonzalez, S., Alcaraz, M. V., Daz, F., Flotte, T. J., De Vargas, I. F., Anderson, R. R., & Nik, K. (1997). DNA ploidy changes in rhino mouse skin induced by all-trans retinoic acid and retinol. Skin Pharmacology, 10(3), 135-143.

DNA ploidy changes in rhino mouse skin induced by all-trans retinoic acid and retinol. / Gonzalez, Salvador; Alcaraz, Maria Victoria; Daz, Florentina; Flotte, Thomas J; De Vargas, Ignacio Ferez; Anderson, R. Rox; Nik, Kollias.

In: Skin Pharmacology, Vol. 10, No. 3, 1997, p. 135-143.

Research output: Contribution to journalArticle

Gonzalez, S, Alcaraz, MV, Daz, F, Flotte, TJ, De Vargas, IF, Anderson, RR & Nik, K 1997, 'DNA ploidy changes in rhino mouse skin induced by all-trans retinoic acid and retinol', Skin Pharmacology, vol. 10, no. 3, pp. 135-143.
Gonzalez S, Alcaraz MV, Daz F, Flotte TJ, De Vargas IF, Anderson RR et al. DNA ploidy changes in rhino mouse skin induced by all-trans retinoic acid and retinol. Skin Pharmacology. 1997;10(3):135-143.
Gonzalez, Salvador ; Alcaraz, Maria Victoria ; Daz, Florentina ; Flotte, Thomas J ; De Vargas, Ignacio Ferez ; Anderson, R. Rox ; Nik, Kollias. / DNA ploidy changes in rhino mouse skin induced by all-trans retinoic acid and retinol. In: Skin Pharmacology. 1997 ; Vol. 10, No. 3. pp. 135-143.
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abstract = "Objective: In order to assess the proliferative changes induced by all-trans retinoic acid (PA) and retinol (ROL), we have carried out a study of the DNA content of basal and suprabasal keratinocytes after epicutaneous application on the rhino mouse. Study Design: Skin sections were analyzed stereologically and cytophotometrically using the Feulgen technique. The diploid DNA value (2C) was obtained from hepatocyte nuclei of control animals. Whereas cells in phase G0-G1 will show a 2C content, cells during phase S and in phase G2-M will show DNA values ranging from 2C to 4C and 4C, respectively. Results: Although epidermal thickness (ET) increased significantly in all treated animals, surface density only increased in animals treated with all-trans RA. Quantification of DNA content of basal keratinocytes showed reduction of 2C and 2C-4C populations with a commensurate increase in proportions of cells with 4C and > 4C in the animals treated with 0.025{\%} all-trans RA and ROL. Suprabasal keratinocytes of mice treated with 0.025{\%} all-trans showed a decrease of the 2C population and an increased proportion of cells with 4C. Whereas 0.025{\%} all-trans RA induced an increase of both basal and suprabasal DNA indices, ROL enhanced only the basal DNA index significantly. Conclusion: Animals treated with 0.025{\%} ROL showed a significant increase in the basal proliferative index (PI) while the suprabasal PI remained constant; treatment with 0.025{\%} all-trans RA produced a significant increase of both basal and suprabasal PIs and parakeratotic hyperkeratosis probably due to incomplete differentiation.",
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AU - Gonzalez, Salvador

AU - Alcaraz, Maria Victoria

AU - Daz, Florentina

AU - Flotte, Thomas J

AU - De Vargas, Ignacio Ferez

AU - Anderson, R. Rox

AU - Nik, Kollias

PY - 1997

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N2 - Objective: In order to assess the proliferative changes induced by all-trans retinoic acid (PA) and retinol (ROL), we have carried out a study of the DNA content of basal and suprabasal keratinocytes after epicutaneous application on the rhino mouse. Study Design: Skin sections were analyzed stereologically and cytophotometrically using the Feulgen technique. The diploid DNA value (2C) was obtained from hepatocyte nuclei of control animals. Whereas cells in phase G0-G1 will show a 2C content, cells during phase S and in phase G2-M will show DNA values ranging from 2C to 4C and 4C, respectively. Results: Although epidermal thickness (ET) increased significantly in all treated animals, surface density only increased in animals treated with all-trans RA. Quantification of DNA content of basal keratinocytes showed reduction of 2C and 2C-4C populations with a commensurate increase in proportions of cells with 4C and > 4C in the animals treated with 0.025% all-trans RA and ROL. Suprabasal keratinocytes of mice treated with 0.025% all-trans showed a decrease of the 2C population and an increased proportion of cells with 4C. Whereas 0.025% all-trans RA induced an increase of both basal and suprabasal DNA indices, ROL enhanced only the basal DNA index significantly. Conclusion: Animals treated with 0.025% ROL showed a significant increase in the basal proliferative index (PI) while the suprabasal PI remained constant; treatment with 0.025% all-trans RA produced a significant increase of both basal and suprabasal PIs and parakeratotic hyperkeratosis probably due to incomplete differentiation.

AB - Objective: In order to assess the proliferative changes induced by all-trans retinoic acid (PA) and retinol (ROL), we have carried out a study of the DNA content of basal and suprabasal keratinocytes after epicutaneous application on the rhino mouse. Study Design: Skin sections were analyzed stereologically and cytophotometrically using the Feulgen technique. The diploid DNA value (2C) was obtained from hepatocyte nuclei of control animals. Whereas cells in phase G0-G1 will show a 2C content, cells during phase S and in phase G2-M will show DNA values ranging from 2C to 4C and 4C, respectively. Results: Although epidermal thickness (ET) increased significantly in all treated animals, surface density only increased in animals treated with all-trans RA. Quantification of DNA content of basal keratinocytes showed reduction of 2C and 2C-4C populations with a commensurate increase in proportions of cells with 4C and > 4C in the animals treated with 0.025% all-trans RA and ROL. Suprabasal keratinocytes of mice treated with 0.025% all-trans showed a decrease of the 2C population and an increased proportion of cells with 4C. Whereas 0.025% all-trans RA induced an increase of both basal and suprabasal DNA indices, ROL enhanced only the basal DNA index significantly. Conclusion: Animals treated with 0.025% ROL showed a significant increase in the basal proliferative index (PI) while the suprabasal PI remained constant; treatment with 0.025% all-trans RA produced a significant increase of both basal and suprabasal PIs and parakeratotic hyperkeratosis probably due to incomplete differentiation.

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