Abstract
Objective: In order to assess the proliferative changes induced by all-trans retinoic acid (RA) and retinol (ROL), we have carried out a study of the DNA content of basal and suprabasal keratinocytes after epicutaneous application on the rhino mouse. Study Design: Skin sections were analyzed ste-reologically and cytophotometrically using the Feulgen technique. The diploid DNA value (2C) was obtained from hepa-tocyte nuclei of control animals. Whereas cells inphase G0-G1 will show a 2C content, cells during phase S and in phase G2-M will show DNA values ranging from 2C to 4C and 4C, respectively. Results: Although epidermal thickness (ET) increased significantly in all treated animals, surface density only increased in animals treated with all-trans RA. Quantification of DNAcontent of basal keratinocytes showed reduction of 2C and 2C-4C populations with a commensurate increase in proportions of cells with 4C and > 4C in the animals treated with 0.025% all-trans RA and ROL. Suprabasal keratinocytes of mice treated with 0.025% all-trans showed a decrease of the 2C population and an increased proportion of cells with 4C. Whereas 0.025% all-trans RA induced an increase of both basal and suprabasal DNA indices, ROL enhanced only the basal DNAindex significantly. Conclusion: Animals treated with 0.025% ROL showed a significant increase in the basal proliferative index (PI) while the suprabasal PI remained constant; treatment with 0.025% all-trans RA produced a significant increase of both basal and suprabasal PIs and parakeratotic hyperkeratosis probably due to incomplete differentiation.
Original language | English (US) |
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Pages (from-to) | 135-143 |
Number of pages | 9 |
Journal | Skin Pharmacology and Physiology |
Volume | 10 |
Issue number | 3 |
DOIs | |
State | Published - Jan 1 1997 |
Keywords
- All-trans retinoic acid
- DNA content
- Epidermal cell kinetics
- Retinol
- Rhino mouse
ASJC Scopus subject areas
- Physiology
- Pharmacology
- Dermatology