DNA methylation profile of liver tissue in end-stage cholestatic liver disease

Angela C. Cheung, Brian D. Juran, Erik M. Schlicht, Bryan M. McCauley, Elizabeth J. Atkinson, Raymond Moore, Julie Heimbach, Kymberly D. Watt, Tsung Teh Wu, Nicholas F. Larusso, Gregory J. Gores, Zhifu Sun, Konstantinos N. Lazaridis

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: In this methylome-wide association study of cholestatic liver diseases (primary sclerosing cholangitis and primary biliary cholangitis), the authors aimed to elucidate changes in methylome and pathway enrichment to identify candidate genes. Patients methods: Reduced representation bisulfite sequencing was performed on liver tissue from 58 patients with primary sclerosing cholangitis (n = 13), primary biliary cholangitis (n = 20), alcoholic liver disease (n = 21) and live liver donors (n = 4). Pathway enrichment and network analysis were used to explore key genes/pathways. Results: Both cholestatic liver diseases were characterized by global hypomethylation, with pathway enrichment demonstrating distinct genes and pathways associated with the methylome. Conclusions: This novel study demonstrated that differential methylation in cholestatic liver disease was associated with unique pathways, suggesting it may drive disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)481-497
Number of pages17
JournalEpigenomics
Volume14
Issue number8
DOIs
StatePublished - Apr 2022

Keywords

  • DNA methylation
  • alcoholic liver disease
  • alcoholic steatohepatitis
  • cirrhosis
  • differentially methylated regions
  • epigenetic
  • methylome
  • primary biliary cholangitis
  • primary biliary cirrhosis
  • primary sclerosing cholangitis

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Fingerprint

Dive into the research topics of 'DNA methylation profile of liver tissue in end-stage cholestatic liver disease'. Together they form a unique fingerprint.

Cite this