DNA-mediated fluctuations in ionic current through silicon oxide nanopore channels

H. Chang, Farhad Kosari, G. Andreadakis, M. A. Alam, George Vasmatzis, R. Bashir

Research output: Contribution to journalArticle

284 Citations (Scopus)

Abstract

Single molecule sensors in which nanoscale pores within biological or artificial membranes act as mechanical gating elements are very promising devices for the rapid characterization and sequencing of nucleic acid molecules. The two terminal electrical measurements of translocation of polymers through single ion channels and that of ssDNA molecules through protein channels have been demonstrated, and have sparked tremendous interest in such single molecule sensors. The prevailing view regarding the nanopore sensors is that there exists no electrical interaction between the nanopore and the translocating molecule, and that all nanopore sensors reported to-date, whether biological or artificial, operate as a coulter-counter, i.e., the ionic current measured across the pore decreases (is mechanically blocked) when the DNA molecule transverses through the pore. We have fabricated nanopore "channel" sensors with a silicon oxide inner surface, and our results challenge the prevailing view of exclusive mechanical interaction during the translocation of dsDNA molecules through these channels. We demonstrate that the ionic current can actually increase due to electrical gating of surface current in the channel due to the charge on the DNA itself.

Original languageEnglish (US)
Pages (from-to)1551-1556
Number of pages6
JournalNano Letters
Volume4
Issue number8
DOIs
StatePublished - Aug 2004

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Nanopores
Silicon oxides
silicon oxides
DNA
deoxyribonucleic acid
Molecules
molecules
sensors
Sensors
porosity
sequencing
Nucleic acids
nucleic acids
Ion Channels
Nucleic Acids
electrical measurement
Polymers
counters
interactions
membranes

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

DNA-mediated fluctuations in ionic current through silicon oxide nanopore channels. / Chang, H.; Kosari, Farhad; Andreadakis, G.; Alam, M. A.; Vasmatzis, George; Bashir, R.

In: Nano Letters, Vol. 4, No. 8, 08.2004, p. 1551-1556.

Research output: Contribution to journalArticle

Chang, H. ; Kosari, Farhad ; Andreadakis, G. ; Alam, M. A. ; Vasmatzis, George ; Bashir, R. / DNA-mediated fluctuations in ionic current through silicon oxide nanopore channels. In: Nano Letters. 2004 ; Vol. 4, No. 8. pp. 1551-1556.
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