TY - JOUR
T1 - DNA end resection and its role in DNA replication and DSB repair choice in mammalian cells
AU - Zhao, Fei
AU - Kim, Wootae
AU - Kloeber, Jake A.
AU - Lou, Zhenkun
N1 - Funding Information:
This work was supported by the National Institutes of Health [RO1CA203971 and RO1CA203561 to Z.L.]. J.A.K. was supported by T32GM65841.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/10
Y1 - 2020/10
N2 - DNA end resection has a key role in double-strand break repair and DNA replication. Defective DNA end resection can cause malfunctions in DNA repair and replication, leading to greater genomic instability. DNA end resection is initiated by MRN-CtIP generating short, 3′-single-stranded DNA (ssDNA). This newly generated ssDNA is further elongated by multiple nucleases and DNA helicases, such as EXO1, DNA2, and BLM. Effective DNA end resection is essential for error-free homologous recombination DNA repair, the degradation of incorrectly replicated DNA and double-strand break repair choice. Because of its importance in DNA repair, DNA end resection is strictly regulated. Numerous mechanisms have been reported to regulate the initiation, extension, and termination of DNA end resection. Here, we review the general process of DNA end resection and its role in DNA replication and repair pathway choice.
AB - DNA end resection has a key role in double-strand break repair and DNA replication. Defective DNA end resection can cause malfunctions in DNA repair and replication, leading to greater genomic instability. DNA end resection is initiated by MRN-CtIP generating short, 3′-single-stranded DNA (ssDNA). This newly generated ssDNA is further elongated by multiple nucleases and DNA helicases, such as EXO1, DNA2, and BLM. Effective DNA end resection is essential for error-free homologous recombination DNA repair, the degradation of incorrectly replicated DNA and double-strand break repair choice. Because of its importance in DNA repair, DNA end resection is strictly regulated. Numerous mechanisms have been reported to regulate the initiation, extension, and termination of DNA end resection. Here, we review the general process of DNA end resection and its role in DNA replication and repair pathway choice.
UR - http://www.scopus.com/inward/record.url?scp=85094193140&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85094193140&partnerID=8YFLogxK
U2 - 10.1038/s12276-020-00519-1
DO - 10.1038/s12276-020-00519-1
M3 - Review article
C2 - 33122806
AN - SCOPUS:85094193140
SN - 1226-3613
VL - 52
SP - 1705
EP - 1714
JO - Experimental and Molecular Medicine
JF - Experimental and Molecular Medicine
IS - 10
ER -