DNA end resection and its role in DNA replication and DSB repair choice in mammalian cells

Fei Zhao, Wootae Kim, Jake A. Kloeber, Zhenkun Lou

Research output: Contribution to journalReview articlepeer-review

2 Scopus citations

Abstract

DNA end resection has a key role in double-strand break repair and DNA replication. Defective DNA end resection can cause malfunctions in DNA repair and replication, leading to greater genomic instability. DNA end resection is initiated by MRN-CtIP generating short, 3′-single-stranded DNA (ssDNA). This newly generated ssDNA is further elongated by multiple nucleases and DNA helicases, such as EXO1, DNA2, and BLM. Effective DNA end resection is essential for error-free homologous recombination DNA repair, the degradation of incorrectly replicated DNA and double-strand break repair choice. Because of its importance in DNA repair, DNA end resection is strictly regulated. Numerous mechanisms have been reported to regulate the initiation, extension, and termination of DNA end resection. Here, we review the general process of DNA end resection and its role in DNA replication and repair pathway choice.

Original languageEnglish (US)
Pages (from-to)1705-1714
Number of pages10
JournalExperimental and Molecular Medicine
Volume52
Issue number10
DOIs
StatePublished - Oct 2020

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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