DNA bending by hexamethylene-tethered ammonium ions

J. K. Strauss, C. Roberts, M. G. Nelson, C. Switzer, L James Maher III

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

DNA is bent when complexed with certain proteins. We are exploring the hypothesis that asymmetric neutralization of phosphate charges will cause the DNA double helix to collapse toward the neutralized face. We have previously shown that DNA spontaneously bends toward one face of the double helix when it is partially substituted with neutral methylphosphonate linkages. We have now synthesized DNA duplexes in which cations are tethered by hexamethylene chains near specific phosphates. Electrophoretic phasing experiments demonstrate that tethering six ammonium ions on one helical face causes DNA to bend by ≃5° toward that face, in qualitative agreement with predictions. Ion pairing between tethered cottons and DNA phosphates provides a new model for simulating the electrostatic consequences of phosphate neutralization by proteins.

Original languageEnglish (US)
Pages (from-to)9515-9520
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number18
DOIs
StatePublished - Sep 3 1996

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Ammonium Compounds
Ions
DNA
Phosphates
Static Electricity
Cations
Proteins

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

DNA bending by hexamethylene-tethered ammonium ions. / Strauss, J. K.; Roberts, C.; Nelson, M. G.; Switzer, C.; Maher III, L James.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 18, 03.09.1996, p. 9515-9520.

Research output: Contribution to journalArticle

Strauss, J. K. ; Roberts, C. ; Nelson, M. G. ; Switzer, C. ; Maher III, L James. / DNA bending by hexamethylene-tethered ammonium ions. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 18. pp. 9515-9520.
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