Distribution of interstitial cells of Cajal and nitrergic neurons in normal and diabetic human appendix

S. M. Miller, R. A. Narasimhan, P. F. Schmalz, E. E. Soffer, R. M. Walsh, V. Krishnamurthi, P. J. Pasricha, J. H. Szurszewski, Gianrico Farrugia

Research output: Contribution to journalArticle

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Abstract

The objective of this study was to determine the distribution of enteric nerves and interstitial cells of Cajal (ICC) in the normal human appendix and in type 1 diabetes. Appendixes were collected from patients with type 1 diabetes and from non-diabetic controls. Volumes of nerves and ICC were determined using 3-D reconstruction and neuronal nitric oxide synthase (nNOS) expressing neurons were counted. Enteric ganglia were found in the myenteric plexus region and within the longitudinal muscle. ICC were found throughout the muscle layers. In diabetes, c-Kit positive ICC volumes were significantly reduced as were nNOS expressing neurons. In conclusion, we describe the distribution of ICC and enteric nerves in health and in diabetes. The data also suggest that the human appendix, a readily available source of human tissue, may be useful model for the study of motility disorders.

Original languageEnglish (US)
Pages (from-to)349-357
Number of pages9
JournalNeurogastroenterology and Motility
Volume20
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Nitrergic Neurons
Interstitial Cells of Cajal
Neurons
Nitric Oxide Synthase Type I
Type 1 Diabetes Mellitus
Myenteric Plexus
Muscles
Cell Size
Ganglia
Health

Keywords

  • c-Kit
  • Fibrosis
  • Myenteric plexus
  • Neuronal nitric oxide synthase
  • PGP 9.5
  • Smooth muscle

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Neuroscience(all)

Cite this

Distribution of interstitial cells of Cajal and nitrergic neurons in normal and diabetic human appendix. / Miller, S. M.; Narasimhan, R. A.; Schmalz, P. F.; Soffer, E. E.; Walsh, R. M.; Krishnamurthi, V.; Pasricha, P. J.; Szurszewski, J. H.; Farrugia, Gianrico.

In: Neurogastroenterology and Motility, Vol. 20, No. 4, 04.2008, p. 349-357.

Research output: Contribution to journalArticle

Miller, SM, Narasimhan, RA, Schmalz, PF, Soffer, EE, Walsh, RM, Krishnamurthi, V, Pasricha, PJ, Szurszewski, JH & Farrugia, G 2008, 'Distribution of interstitial cells of Cajal and nitrergic neurons in normal and diabetic human appendix', Neurogastroenterology and Motility, vol. 20, no. 4, pp. 349-357. https://doi.org/10.1111/j.1365-2982.2007.01040.x
Miller, S. M. ; Narasimhan, R. A. ; Schmalz, P. F. ; Soffer, E. E. ; Walsh, R. M. ; Krishnamurthi, V. ; Pasricha, P. J. ; Szurszewski, J. H. ; Farrugia, Gianrico. / Distribution of interstitial cells of Cajal and nitrergic neurons in normal and diabetic human appendix. In: Neurogastroenterology and Motility. 2008 ; Vol. 20, No. 4. pp. 349-357.
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