Distinctive expression and functions of the type 4 endothelial differentiation gene-encoded G protein-coupled receptor for lysophosphatidic acid in ovarian cancer

Edward J. Goetzl, Hana Dolezalova, Yvonne Kong, Yu Long Hu, Robert B. Jaffe, Kimberly R. Kalli, Cheryl A Conover

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Endothelial differentiation gene (edg)-encoded G protein-coupled receptors (Edg Rs)-1, -3, and -5 bind sphingosine 1-phosphate (S1P), and Edg- 2 and -4 bind lysophosphatidic acid (LPA). Edg Rs transduce signals from LPA and S1P that stimulate ras- and rho-dependent cellular proliferation, enhance cellular survival, and suppress apoptosis. That high levels of LPA in plasma and ascitic fluid of patients with ovarian cancer correlate with widespread invasion suggested the importance of investigating expression and functions of Edg Rs in ovarian cancer cells (OCCs) as compared with nonmalignant ovarian surface epithelial cells (OSEs). Analyses of Edg Rs by semiquantitative reverse transcription-PCR, a radioactively quantified variant of PCR, and Western blots developed with monoclonal antibodies showed prominent expression of Edg-4 R in primary cultures and established lines of OCCs but none in OSEs. In contrast, levels of Edg-2, -3, and -5 were higher in OSEs than OCCs. LPA stimulated proliferation and signaled a serum response element-luciferase reporter of immediate-early gene activation in OCCs but not OSEs, whereas SIP evoked similar responses in both OSEs and OCCs. Pharmacological inhibitors of Edg R signaling suppressed OCC responses to LPA. A combination of monoclonal anti-Edg-4 R antibody and phorbol myristate acetate, which were inactive separately, evoked proliferative and serum response element-luciferase responses of OCCs but not OSEs. Thus the Edg-4 R may represent a distinctive marker of OCC that transduces growth-promoting signals from the high local concentrations of LPA characteristic of aggressive ovarian cancer.

Original languageEnglish (US)
Pages (from-to)5370-5375
Number of pages6
JournalCancer Research
Volume59
Issue number20
StatePublished - Oct 15 1999

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G-Protein-Coupled Receptors
Ovarian Neoplasms
Epithelial Cells
Genes
Serum Response Element
Luciferases
Polymerase Chain Reaction
Immediate-Early Genes
lysophosphatidic acid
Ascitic Fluid
Tetradecanoylphorbol Acetate
Transcriptional Activation
Reverse Transcription
Western Blotting
Monoclonal Antibodies
Cell Proliferation
Pharmacology
Apoptosis
Survival
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Distinctive expression and functions of the type 4 endothelial differentiation gene-encoded G protein-coupled receptor for lysophosphatidic acid in ovarian cancer. / Goetzl, Edward J.; Dolezalova, Hana; Kong, Yvonne; Hu, Yu Long; Jaffe, Robert B.; Kalli, Kimberly R.; Conover, Cheryl A.

In: Cancer Research, Vol. 59, No. 20, 15.10.1999, p. 5370-5375.

Research output: Contribution to journalArticle

Goetzl, Edward J. ; Dolezalova, Hana ; Kong, Yvonne ; Hu, Yu Long ; Jaffe, Robert B. ; Kalli, Kimberly R. ; Conover, Cheryl A. / Distinctive expression and functions of the type 4 endothelial differentiation gene-encoded G protein-coupled receptor for lysophosphatidic acid in ovarian cancer. In: Cancer Research. 1999 ; Vol. 59, No. 20. pp. 5370-5375.
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abstract = "Endothelial differentiation gene (edg)-encoded G protein-coupled receptors (Edg Rs)-1, -3, and -5 bind sphingosine 1-phosphate (S1P), and Edg- 2 and -4 bind lysophosphatidic acid (LPA). Edg Rs transduce signals from LPA and S1P that stimulate ras- and rho-dependent cellular proliferation, enhance cellular survival, and suppress apoptosis. That high levels of LPA in plasma and ascitic fluid of patients with ovarian cancer correlate with widespread invasion suggested the importance of investigating expression and functions of Edg Rs in ovarian cancer cells (OCCs) as compared with nonmalignant ovarian surface epithelial cells (OSEs). Analyses of Edg Rs by semiquantitative reverse transcription-PCR, a radioactively quantified variant of PCR, and Western blots developed with monoclonal antibodies showed prominent expression of Edg-4 R in primary cultures and established lines of OCCs but none in OSEs. In contrast, levels of Edg-2, -3, and -5 were higher in OSEs than OCCs. LPA stimulated proliferation and signaled a serum response element-luciferase reporter of immediate-early gene activation in OCCs but not OSEs, whereas SIP evoked similar responses in both OSEs and OCCs. Pharmacological inhibitors of Edg R signaling suppressed OCC responses to LPA. A combination of monoclonal anti-Edg-4 R antibody and phorbol myristate acetate, which were inactive separately, evoked proliferative and serum response element-luciferase responses of OCCs but not OSEs. Thus the Edg-4 R may represent a distinctive marker of OCC that transduces growth-promoting signals from the high local concentrations of LPA characteristic of aggressive ovarian cancer.",
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