Differential expression of thyroid transcription factor 1 in small cell lung carcinoma and Merkel cell tumor

Angela L. Byrd-Gloster; Andras Khoor; Lewis F. Glass; Jane L. Messina; Jeffrey A. Whitsett; Sandra K. Livingston; Philip T. Cagle

doi:10.1016/S0046-8177(00)80199-9

Differential expression of thyroid transcription factor 1 in small cell lung carcinoma and Merkel cell tumor

Angela L. Byrd-Gloster, Andras Khoor, Lewis F. Glass, Jane L. Messina, Jeffrey A. Whitsett, Sandra K. Livingston, Philip T. Cagle

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

115 Scopus citations

Abstract

The distinction between metastatic small cell lung carcinoma (SCLC) and Merkel cell tumor is difficult by routine histology, prompting the search for specific markers that could separate these neoplasms. Thyroid transcription factor 1 (TFF-1) is a homeodomain containing transcription factor expressed in the normal airway epithelium. The expression of TTF-1 has also been shown in adenocarcinomas and small cell carcinomas of the lung. However, the utility of TTF-1 to differentiate between SCLC and Merkel cell tumor has not yet been investigated. In this study, paraffin sections of 36 SCLCs and 21 Merkel cell tumors were analyzed for the presence of immunoreactive TTF-1 and cytokeratin 20 (CK20), a marker previously demonstrated in Merkel cell tumors. Monoclonal TTF-1 and CK20 antibodies were used with a biotin- streptavidin detection system. Immunostaining for TTF-1 was observed in 97% of SCLCs and in no Merkel cell tumors. Immunoreactivity for CK20 was demonstrated in 76% of Merkel cell tumors and 3% of SCLCs. These data indicate that TTF-1 is a sensitive (97%) and specific (100%) marker for SCLCs and can be used to differentiate SCLCs from Merkel cell tumors.

Original language	English (US)
Pages (from-to)	58-62
Number of pages	5
Journal	Human Pathology
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/S0046-8177(00)80199-9
State	Published - 2000

Keywords

CK20
Immunohistochemistry
Merkel cell tumor
Small cell lung carcinoma
TTF- 1

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/S0046-8177(00)80199-9

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title = "Differential expression of thyroid transcription factor 1 in small cell lung carcinoma and Merkel cell tumor",

abstract = "The distinction between metastatic small cell lung carcinoma (SCLC) and Merkel cell tumor is difficult by routine histology, prompting the search for specific markers that could separate these neoplasms. Thyroid transcription factor 1 (TFF-1) is a homeodomain containing transcription factor expressed in the normal airway epithelium. The expression of TTF-1 has also been shown in adenocarcinomas and small cell carcinomas of the lung. However, the utility of TTF-1 to differentiate between SCLC and Merkel cell tumor has not yet been investigated. In this study, paraffin sections of 36 SCLCs and 21 Merkel cell tumors were analyzed for the presence of immunoreactive TTF-1 and cytokeratin 20 (CK20), a marker previously demonstrated in Merkel cell tumors. Monoclonal TTF-1 and CK20 antibodies were used with a biotin- streptavidin detection system. Immunostaining for TTF-1 was observed in 97% of SCLCs and in no Merkel cell tumors. Immunoreactivity for CK20 was demonstrated in 76% of Merkel cell tumors and 3% of SCLCs. These data indicate that TTF-1 is a sensitive (97%) and specific (100%) marker for SCLCs and can be used to differentiate SCLCs from Merkel cell tumors.",

keywords = "CK20, Immunohistochemistry, Merkel cell tumor, Small cell lung carcinoma, TTF- 1",

author = "Byrd-Gloster, {Angela L.} and Andras Khoor and Glass, {Lewis F.} and Messina, {Jane L.} and Whitsett, {Jeffrey A.} and Livingston, {Sandra K.} and Cagle, {Philip T.}",

note = "Funding Information: From the H. Lee Moffitt Cancer Center and the University of South Florida College of Medicine, Tampa, FL: Children's Hospital. Cincinnati. OH; and Baylor College of Medicine. Houston. IX. Accepted for publication September 23. 1999. Supported in part by Grants HL 56387 and HL 38859 (JAW1 from the National Institutes of Health, Bethesda, MD. Address correspondence and reprint requests to Andras Khoor. MD. H. Lee Moffitt Cancer Center at the University of South Florida. Department of Pathology, 12902 Magnolia Dr, Tampa, FL 33612-9497. Copyright {\textcopyright} 2000 by W.B. Saunders Company 0046-8177/00/3101-0010510.00/0",

year = "2000",

doi = "10.1016/S0046-8177(00)80199-9",

language = "English (US)",

volume = "31",

pages = "58--62",

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T1 - Differential expression of thyroid transcription factor 1 in small cell lung carcinoma and Merkel cell tumor

AU - Byrd-Gloster, Angela L.

AU - Khoor, Andras

AU - Glass, Lewis F.

AU - Messina, Jane L.

AU - Whitsett, Jeffrey A.

AU - Livingston, Sandra K.

AU - Cagle, Philip T.

N1 - Funding Information: From the H. Lee Moffitt Cancer Center and the University of South Florida College of Medicine, Tampa, FL: Children's Hospital. Cincinnati. OH; and Baylor College of Medicine. Houston. IX. Accepted for publication September 23. 1999. Supported in part by Grants HL 56387 and HL 38859 (JAW1 from the National Institutes of Health, Bethesda, MD. Address correspondence and reprint requests to Andras Khoor. MD. H. Lee Moffitt Cancer Center at the University of South Florida. Department of Pathology, 12902 Magnolia Dr, Tampa, FL 33612-9497. Copyright © 2000 by W.B. Saunders Company 0046-8177/00/3101-0010510.00/0

PY - 2000

Y1 - 2000

N2 - The distinction between metastatic small cell lung carcinoma (SCLC) and Merkel cell tumor is difficult by routine histology, prompting the search for specific markers that could separate these neoplasms. Thyroid transcription factor 1 (TFF-1) is a homeodomain containing transcription factor expressed in the normal airway epithelium. The expression of TTF-1 has also been shown in adenocarcinomas and small cell carcinomas of the lung. However, the utility of TTF-1 to differentiate between SCLC and Merkel cell tumor has not yet been investigated. In this study, paraffin sections of 36 SCLCs and 21 Merkel cell tumors were analyzed for the presence of immunoreactive TTF-1 and cytokeratin 20 (CK20), a marker previously demonstrated in Merkel cell tumors. Monoclonal TTF-1 and CK20 antibodies were used with a biotin- streptavidin detection system. Immunostaining for TTF-1 was observed in 97% of SCLCs and in no Merkel cell tumors. Immunoreactivity for CK20 was demonstrated in 76% of Merkel cell tumors and 3% of SCLCs. These data indicate that TTF-1 is a sensitive (97%) and specific (100%) marker for SCLCs and can be used to differentiate SCLCs from Merkel cell tumors.

AB - The distinction between metastatic small cell lung carcinoma (SCLC) and Merkel cell tumor is difficult by routine histology, prompting the search for specific markers that could separate these neoplasms. Thyroid transcription factor 1 (TFF-1) is a homeodomain containing transcription factor expressed in the normal airway epithelium. The expression of TTF-1 has also been shown in adenocarcinomas and small cell carcinomas of the lung. However, the utility of TTF-1 to differentiate between SCLC and Merkel cell tumor has not yet been investigated. In this study, paraffin sections of 36 SCLCs and 21 Merkel cell tumors were analyzed for the presence of immunoreactive TTF-1 and cytokeratin 20 (CK20), a marker previously demonstrated in Merkel cell tumors. Monoclonal TTF-1 and CK20 antibodies were used with a biotin- streptavidin detection system. Immunostaining for TTF-1 was observed in 97% of SCLCs and in no Merkel cell tumors. Immunoreactivity for CK20 was demonstrated in 76% of Merkel cell tumors and 3% of SCLCs. These data indicate that TTF-1 is a sensitive (97%) and specific (100%) marker for SCLCs and can be used to differentiate SCLCs from Merkel cell tumors.

KW - CK20

KW - Immunohistochemistry

KW - Merkel cell tumor

KW - Small cell lung carcinoma

KW - TTF- 1

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DO - 10.1016/S0046-8177(00)80199-9

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C2 - 10665914

AN - SCOPUS:0033958853

SN - 0046-8177

VL - 31

SP - 58

EP - 62

JO - Human Pathology

JF - Human Pathology

IS - 1

ER -

Differential expression of thyroid transcription factor 1 in small cell lung carcinoma and Merkel cell tumor

Abstract

Keywords

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