Differential expression of nuclear envelope lamins A and C in human lung cancer cell lines

Scott H Kaufmann, Mack Mabry, Rajani Jasti, Joel H. Shaper

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The lamins, an intranuclear class of intermediate filament proteins, are major structural proteins of the nuclear envelope. In the present study, the three abundant mammalian lamins (lamins A, B, and C) were observed to be present in roughly equivalent amounts in the Calu-1, Calu-3, H157, and SK-MES-1 non-small cell lung cancer lines. In the small cell lung cancer lines OH-1, OH-3, NCI-H82, NCI-H209, and NCI-H249, levels of lamin B were similar to those observed in the non-small cell lines, but the levels of lamins A and C were diminished by ≥80%. The relationship between lung cancer phenotype and lamin expression was explored further in the NCI-H249 small cell line. Introduction of the v-rasH oncogene into this line gives rise to a cell line (NCI-H249rasH) with many features of large cell carcinoma of the lung (Falco, J. P., Baylin, S. B., Lupu, R., et al. J. Clin. Invest., 85: 1740-1745, 1990). Concomitant with the v-rasH-induced change in phenotype, a >10-fold increase in the amounts of lamins A and C was observed. Levels of the cytoplasmic intermediate filament protein vimentin also increased. In contrast, levels of a variety of nonlamin nuclear polypeptides including topoisomerase I, topoisomerase II, poly(ADP-ribose) polymerase, and the nucleolar protein B23/nucleophosmin did not change. Comparison of polyadenylated RNA from NCI-H249 and NCI-H249rasH cells on Northern blots revealed similar levels of the mRNA for lamin B but higher levels of the mRNAs for lamins A and C in the v-rasH-expressing cell line. These observations provide evidence for differences in nuclear envelope structure in histologically different neoplastic cells derived from the same epithelial cell system and suggest that differences in lamina structure result from phenotype-specific differences in lamin gene expression.

Original languageEnglish (US)
Pages (from-to)581-586
Number of pages6
JournalCancer Research
Volume51
Issue number2
StatePublished - Jan 15 1991
Externally publishedYes

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Lamins
Lamin Type A
Lamin Type B
Nuclear Envelope
Lung Neoplasms
Exanthema
Intermediate Filament Proteins
Cell Line
Phenotype
Messenger RNA
Large Cell Carcinoma
Type II DNA Topoisomerase
Type I DNA Topoisomerase
Poly(ADP-ribose) Polymerases
Small Cell Lung Carcinoma
Vimentin
Cytoskeleton
Oncogenes
Non-Small Cell Lung Carcinoma
Northern Blotting

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Differential expression of nuclear envelope lamins A and C in human lung cancer cell lines. / Kaufmann, Scott H; Mabry, Mack; Jasti, Rajani; Shaper, Joel H.

In: Cancer Research, Vol. 51, No. 2, 15.01.1991, p. 581-586.

Research output: Contribution to journalArticle

Kaufmann, Scott H ; Mabry, Mack ; Jasti, Rajani ; Shaper, Joel H. / Differential expression of nuclear envelope lamins A and C in human lung cancer cell lines. In: Cancer Research. 1991 ; Vol. 51, No. 2. pp. 581-586.
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abstract = "The lamins, an intranuclear class of intermediate filament proteins, are major structural proteins of the nuclear envelope. In the present study, the three abundant mammalian lamins (lamins A, B, and C) were observed to be present in roughly equivalent amounts in the Calu-1, Calu-3, H157, and SK-MES-1 non-small cell lung cancer lines. In the small cell lung cancer lines OH-1, OH-3, NCI-H82, NCI-H209, and NCI-H249, levels of lamin B were similar to those observed in the non-small cell lines, but the levels of lamins A and C were diminished by ≥80{\%}. The relationship between lung cancer phenotype and lamin expression was explored further in the NCI-H249 small cell line. Introduction of the v-rasH oncogene into this line gives rise to a cell line (NCI-H249rasH) with many features of large cell carcinoma of the lung (Falco, J. P., Baylin, S. B., Lupu, R., et al. J. Clin. Invest., 85: 1740-1745, 1990). Concomitant with the v-rasH-induced change in phenotype, a >10-fold increase in the amounts of lamins A and C was observed. Levels of the cytoplasmic intermediate filament protein vimentin also increased. In contrast, levels of a variety of nonlamin nuclear polypeptides including topoisomerase I, topoisomerase II, poly(ADP-ribose) polymerase, and the nucleolar protein B23/nucleophosmin did not change. Comparison of polyadenylated RNA from NCI-H249 and NCI-H249rasH cells on Northern blots revealed similar levels of the mRNA for lamin B but higher levels of the mRNAs for lamins A and C in the v-rasH-expressing cell line. These observations provide evidence for differences in nuclear envelope structure in histologically different neoplastic cells derived from the same epithelial cell system and suggest that differences in lamina structure result from phenotype-specific differences in lamin gene expression.",
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