TY - JOUR
T1 - Differential expression of complement receptors on human basophils and mast cells
T2 - Evidence for mast cell heterogeneity and CD88/C5aR expression on skin mast cells
AU - Füreder, Wolfgang
AU - Agis, Hermine
AU - Willheim, Martin
AU - Bankl, Hans C.
AU - Maier, Ulrich
AU - Kishi, Kenji
AU - Müller, Michael R.
AU - Czerwenka, Klaus
AU - Radaszkiewicz, Thaddäus
AU - Butterfield, Joseph H.
AU - Klappacher, Günter W.
AU - Sperr, Wolfgang R.
AU - Oppermann, Martin
AU - Lechner, Klaus
AU - Valent, Peter
PY - 1995
Y1 - 1995
N2 - Complement-dependent activation of immune cells is regulated by cell surface membrane receptors. In this study, expression of complement receptors (CR) on human blood basophils (n = 11), tissue mast cells (lung, n = 7; skin, n = 10; uterus, n = 4; tonsil, n = 3; heart, n = 10), and on respective human cell lines (basophil line KU-812, mast cell line HMC-1) was analyzed by the use of mAbs and indirect immunofluorescence. Normal blood basophils and KU- 812 cells were found to express C5aR (CD88), membrane cofactor protein (CD46), decay-accelerating factor (CD55), and membrane attack complex inhibitory factor (CD59), as well as the previously recognized CR1 (CD35), CR3α (CD11b), CR4α (CD11c), and CR3/4β (CD18). Mast cells from all organs as well as HMC-1 cells expressed CD46, CD55, and CD59, but not CD11b, CD21, or CD35. The C5aR (CD88) was detectable on skin mast cells, a subset (5 to 15%) of cardiac mast cells, and on HMC-1 cells, but not on lung, uterus, or tonsillar mast cells (<5%). Moreover, double immunoperoxidase staining (tryptase vs C5aR/CD88) revealed in situ expression of C5aR on skin, but not lung mast cells. Recombinant human (rh) C5a, at 10-10 to 10-7 M, induced secretion of histamine from basophils (rhC5a, 10 M: 53.4 ± 3.1% vs control <5%) and from skin mast cells (rhC5a, 10-8 M: 25.8 ± 16.1% vs control <10% histamine release), but not from other mast cells (rhC5a or control: <10%, p > 0.05). The rhC5a-induced secretion of histamine from basophils and skin mast cells was inhibited by S5/1, a blocking Ab against CD88 (basophils: 37.2% to 75.1%; skin mast cells: 39.2% to 83.9% inhibition p < 0.05). Together, this study shows that a) basophils and mast cells express a different profile of complement receptors, b) C5a-dependent mediator release in skin mast cells and basophils is mediated via CD88, and c) mast cells constitute a heterogenous lineage in terms of expression of the C5a binding site CD88.
AB - Complement-dependent activation of immune cells is regulated by cell surface membrane receptors. In this study, expression of complement receptors (CR) on human blood basophils (n = 11), tissue mast cells (lung, n = 7; skin, n = 10; uterus, n = 4; tonsil, n = 3; heart, n = 10), and on respective human cell lines (basophil line KU-812, mast cell line HMC-1) was analyzed by the use of mAbs and indirect immunofluorescence. Normal blood basophils and KU- 812 cells were found to express C5aR (CD88), membrane cofactor protein (CD46), decay-accelerating factor (CD55), and membrane attack complex inhibitory factor (CD59), as well as the previously recognized CR1 (CD35), CR3α (CD11b), CR4α (CD11c), and CR3/4β (CD18). Mast cells from all organs as well as HMC-1 cells expressed CD46, CD55, and CD59, but not CD11b, CD21, or CD35. The C5aR (CD88) was detectable on skin mast cells, a subset (5 to 15%) of cardiac mast cells, and on HMC-1 cells, but not on lung, uterus, or tonsillar mast cells (<5%). Moreover, double immunoperoxidase staining (tryptase vs C5aR/CD88) revealed in situ expression of C5aR on skin, but not lung mast cells. Recombinant human (rh) C5a, at 10-10 to 10-7 M, induced secretion of histamine from basophils (rhC5a, 10 M: 53.4 ± 3.1% vs control <5%) and from skin mast cells (rhC5a, 10-8 M: 25.8 ± 16.1% vs control <10% histamine release), but not from other mast cells (rhC5a or control: <10%, p > 0.05). The rhC5a-induced secretion of histamine from basophils and skin mast cells was inhibited by S5/1, a blocking Ab against CD88 (basophils: 37.2% to 75.1%; skin mast cells: 39.2% to 83.9% inhibition p < 0.05). Together, this study shows that a) basophils and mast cells express a different profile of complement receptors, b) C5a-dependent mediator release in skin mast cells and basophils is mediated via CD88, and c) mast cells constitute a heterogenous lineage in terms of expression of the C5a binding site CD88.
UR - http://www.scopus.com/inward/record.url?scp=0029022132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029022132&partnerID=8YFLogxK
M3 - Article
C2 - 7673728
AN - SCOPUS:0029022132
SN - 0022-1767
VL - 155
SP - 3152
EP - 3160
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -