Differential expression of complement receptors on human basophils and mast cells: Evidence for mast cell heterogeneity and CD88/C5aR expression on skin mast cells

W. Fureder, H. Agis, M. Willheim, H. C. Bankl, U. Maier, K. Kishi, M. R. Muller, K. Czerwenka, T. Radaszkiewicz, J. H. Butterfield, G. W. Klappacher, W. R. Sperr, M. Oppermann, K. Lechner, P. Valent

Research output: Contribution to journalArticle

149 Scopus citations

Abstract

Complement-dependent activation of immune cells is regulated by cell surface membrane receptors. In this study, expression of complement receptors (CR) on human blood basophils (n = 11), tissue mast cells (lung, n = 7; skin, n = 10; uterus, n = 4; tonsil, n = 3; heart, n = 10), and on respective human cell lines (basophil line KU-812, mast cell line HMC-1) was analyzed by the use of mAbs and indirect immunofluorescence. Normal blood basophils and KU- 812 cells were found to express C5aR (CD88), membrane cofactor protein (CD46), decay-accelerating factor (CD55), and membrane attack complex inhibitory factor (CD59), as well as the previously recognized CR1 (CD35), CR3α (CD11b), CR4α (CD11c), and CR3/4β (CD18). Mast cells from all organs as well as HMC-1 cells expressed CD46, CD55, and CD59, but not CD11b, CD21, or CD35. The C5aR (CD88) was detectable on skin mast cells, a subset (5 to 15%) of cardiac mast cells, and on HMC-1 cells, but not on lung, uterus, or tonsillar mast cells (<5%). Moreover, double immunoperoxidase staining (tryptase vs C5aR/CD88) revealed in situ expression of C5aR on skin, but not lung mast cells. Recombinant human (rh) C5a, at 10-10 to 10-7 M, induced secretion of histamine from basophils (rhC5a, 10 M: 53.4 ± 3.1% vs control <5%) and from skin mast cells (rhC5a, 10-8 M: 25.8 ± 16.1% vs control <10% histamine release), but not from other mast cells (rhC5a or control: <10%, p > 0.05). The rhC5a-induced secretion of histamine from basophils and skin mast cells was inhibited by S5/1, a blocking Ab against CD88 (basophils: 37.2% to 75.1%; skin mast cells: 39.2% to 83.9% inhibition p < 0.05). Together, this study shows that a) basophils and mast cells express a different profile of complement receptors, b) C5a-dependent mediator release in skin mast cells and basophils is mediated via CD88, and c) mast cells constitute a heterogenous lineage in terms of expression of the C5a binding site CD88.

Original languageEnglish (US)
Pages (from-to)3152-3160
Number of pages9
JournalJournal of Immunology
Volume155
Issue number6
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Fureder, W., Agis, H., Willheim, M., Bankl, H. C., Maier, U., Kishi, K., Muller, M. R., Czerwenka, K., Radaszkiewicz, T., Butterfield, J. H., Klappacher, G. W., Sperr, W. R., Oppermann, M., Lechner, K., & Valent, P. (1995). Differential expression of complement receptors on human basophils and mast cells: Evidence for mast cell heterogeneity and CD88/C5aR expression on skin mast cells. Journal of Immunology, 155(6), 3152-3160.