Differential corticosteroid receptor regulation of mesoaccumbens dopamine efflux during the peak and nadir of the circadian rhythm

A molecular equilibrium in the midbrain?

Susannah J Tye, Anthony D. Miller, Charles D. Blaha

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Corticosteroid receptor modulation of mesoaccumbens dopamine neurotransmission is believed to be a key neurobiological mechanism mediating the effects of stress in addiction. Importantly, nucleus accumbens (NAc) subregions (core and shell) are reported to respond differentially to fluctuating basal levels of glucocorticoids, with dopaminergic responses in the core of the NAc being somewhat impervious to fluctuating levels of glucocorticoids relative to the shell. To investigate the corticosteroid receptor mechanisms mediating basal dopamine efflux in the core of the NAc, we have used chronoamperometry in combination with stearate-modified graphite paste electrodes in urethane anesthetized male Long-Evans rats during the peak and nadir of the circadian cycle. Blockade of ventral tegmental area low-affinity glucocorticoid (GR) or high-affinity mineralocorticoid (MR) receptors with mifepristone (1 μg/μl) or spironolactone (0.2 μg/μl), respectively, indicated that endogenous phase-dependent corticosteroid receptor activation (GRs during peak; MRs during nadir) facilitated extracellular NAc dopamine efflux. Conversely, the alternate receptor's actions appeared inhibitory at these time points (MRs during peak; GRs during nadir). Pharmacological activation of either the GR or MR with corticosterone (2 μg/μl) or aldosterone (0.2 μg/μl), respectively, potentiated NAc dopamine efflux, irrespective of circadian phase. Together, these data suggest that dominant corticosteroid receptor activation stimulates tonic mesoaccumbens dopamine transmission, enabling MRs and GRs to differentially maintain basal NAc dopamine release over the course of the circadian cycle. This points to an important molecular mechanism through which relatively stable NAc core dopamine extracellular levels could be maintained in the face of fluctuating corticosterone circadian rhythms.

Original languageEnglish (US)
Pages (from-to)982-990
Number of pages9
JournalSynapse
Volume63
Issue number11
DOIs
StatePublished - Nov 2009

Fingerprint

Steroid Receptors
Nucleus Accumbens
Mesencephalon
Circadian Rhythm
Dopamine
Glucocorticoids
Corticosterone
Stearates
Mineralocorticoid Receptors
Mifepristone
Mineralocorticoids
Long Evans Rats
Ventral Tegmental Area
Spironolactone
Graphite
Urethane
Ointments
Basal Ganglia
Aldosterone
Synaptic Transmission

Keywords

  • Chronoamperometry
  • Corticosterone
  • Glucocorticoid
  • Mineralocorticoid
  • Ventral tegmentum

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Differential corticosteroid receptor regulation of mesoaccumbens dopamine efflux during the peak and nadir of the circadian rhythm : A molecular equilibrium in the midbrain? / Tye, Susannah J; Miller, Anthony D.; Blaha, Charles D.

In: Synapse, Vol. 63, No. 11, 11.2009, p. 982-990.

Research output: Contribution to journalArticle

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