Different reinnervation patterns in the celiac/mesenteric and superior cervical ganglia following guanethidine sympathectomy in adult rats

Eduardo E. Benarroch, Paula J. Zollman, Inge L. Smithson, James D. Schmelzer, Phillip Anson Low

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We sought to determine whether chronic guanethidine (Gu) treatment in adult rats produces depletion of sympathetic neurons and hyperinnervation by sensory neuropeptides in the celiac/superior mesenteric (C/SMG) ganglion. Rats received Gu 40 mg/kg per day i.p or saline for 5 weeks. Upon completion of treatment, the C/SMG and the superior cervical ganglion (SCG) were examined for neuropeptide Y (NPY), substance P (SP), calcitonin gene-related peptide (CGRP) and vasoactive intestinal polypeptide (VIP), both by immunocytochemistry (ICC) and radioimmunoassay (RIA). Gu produced marked depletion of NPY-containing neurons and NPY content in the C/SMG, similar to that in the SCG (-89 ± 2 vs. -92 ± 4%, respectively). SP and CGRP immunoreactivities were significantly higher iontrol C/SMG as compared with SCG; after Gu treatment, there was no significant increase in either SP or CGRP in the C/SMG, however, both increased in the SCG. In contrast, VIP levels were similar in the SCG and C/SMG in controls and increased in the C/SMG but not in the SCG after Gu treatment. Thus, in adult rats, the C/SMG is as susceptible as the SCG to Gu treatment; the different pattern of hyperinnervation by SP, CGRP and VIP of the C/SMG as compared with the SCG may reflect the different sources for these neuropeptides in prevertebral as compared with paravertebral ganglia.

Original languageEnglish (US)
Pages (from-to)322-326
Number of pages5
JournalBrain Research
Volume644
Issue number2
DOIs
StatePublished - May 2 1994

Fingerprint

Guanethidine
Superior Cervical Ganglion
Sympathectomy
Abdomen
Calcitonin Gene-Related Peptide
Substance P
Neuropeptide Y
Vasoactive Intestinal Peptide
Neuropeptides
Ganglia
Therapeutics
Sensory Receptor Cells
Radioimmunoassay
Immunohistochemistry
Neurons

Keywords

  • Calcitonin gene-related peptide
  • Ganglion
  • Neuropeptide Y
  • Prevertebral
  • Substance P
  • Sympathectomy

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Different reinnervation patterns in the celiac/mesenteric and superior cervical ganglia following guanethidine sympathectomy in adult rats. / Benarroch, Eduardo E.; Zollman, Paula J.; Smithson, Inge L.; Schmelzer, James D.; Low, Phillip Anson.

In: Brain Research, Vol. 644, No. 2, 02.05.1994, p. 322-326.

Research output: Contribution to journalArticle

Benarroch, Eduardo E. ; Zollman, Paula J. ; Smithson, Inge L. ; Schmelzer, James D. ; Low, Phillip Anson. / Different reinnervation patterns in the celiac/mesenteric and superior cervical ganglia following guanethidine sympathectomy in adult rats. In: Brain Research. 1994 ; Vol. 644, No. 2. pp. 322-326.
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