Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats

Cassondra J. Saande, Samantha K. Pritchard, Deanna M. Worrall, Sarah E. Snavely, Caitlyn A. Nass, Joshua C. Neuman, Rebecca A. Luchtel, Sarah Dobiszewski, Joshua W. Miller, Mario Vailati-Riboni, Juan J. Loor, Kevin L. Schalinske

Research output: Contribution to journalArticle

Abstract

Background: Hyperhomocysteinemia is associated with increased cardiovascular disease risk. Whole eggs contain several nutrients known to affect homocysteine regulation, including sulfur amino acids, choline, and B vitamins. Objective: The aim of this study was to determine the effect of whole eggs and egg components (i.e., egg protein and choline) with respect to 1) homocysteine balance and 2) the hepatic expression and activity of betaine-homocysteine S-methyltransferase (BHMT) and cystathionine β-synthase (CBS) in a folate-restricted (FR) rat model of hyperhomocysteinemia. Methods: Male Sprague Dawley rats (n = 48; 6 wk of age) were randomly assigned to a casein-based diet (C; n = 12), a casein-based diet supplemented with choline (C + Cho; 1.3%, wt:wt; n = 12), an egg protein-based diet (EP; n = 12), or a whole egg-based diet (WE; n = 12). At week 2, half of the rats in each of the 4 dietary groups were provided an FR (0 g folic acid/kg) diet and half continued on the folate-sufficient (FS; 0.2 g folic acid/kg) diet for an additional 6 wk. All diets contained 20% (wt:wt) total protein. Serum homocysteine was measured by HPLC and BHMT and CBS expression and activity were evaluated using real-time quantitative polymerase chain reaction, Western blot, and enzyme activity. A 2-factor ANOVA was used for statistical comparisons. Results: Rats fed FR-C exhibited a 53% increase in circulating homocysteine concentrations compared with rats fed FS-C (P < 0.001). In contrast, serum homocysteine did not differ between rats fed FS-C and FR-EP (P = 0.078). Hepatic BHMT activity was increased by 45% and 40% by the EP (P < 0.001) and WE (P = 0.002) diets compared with the C diets, respectively. Conclusions: Dietary intervention with egg protein prevented elevated circulating homocysteine concentrations in a rat model of hyperhomocysteinemia, due in part to upregulation of hepatic BHMT. These data may support the inclusion of egg protein for dietary recommendations targeting hyperhomocysteinemia prevention.

Original languageEnglish (US)
Pages (from-to)1369-1376
Number of pages8
JournalJournal of Nutrition
Volume149
Issue number8
DOIs
StatePublished - Aug 1 2019

Fingerprint

Dietary Egg Proteins
Betaine-Homocysteine S-Methyltransferase
Hyperhomocysteinemia
Folic Acid
Up-Regulation
Homocysteine
Diet
Liver
Egg Proteins
Choline
Caseins
Eggs
Ovum
Cystathionine
Sulfur Amino Acids
Vitamin B Complex
Serum

Keywords

  • betaine-homocysteine S-methyltransferase
  • cardiovascular disease
  • egg protein
  • folate-restricted diet
  • hyperhomocysteinemia
  • rat
  • whole egg

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Saande, C. J., Pritchard, S. K., Worrall, D. M., Snavely, S. E., Nass, C. A., Neuman, J. C., ... Schalinske, K. L. (2019). Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats. Journal of Nutrition, 149(8), 1369-1376. https://doi.org/10.1093/jn/nxz069

Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats. / Saande, Cassondra J.; Pritchard, Samantha K.; Worrall, Deanna M.; Snavely, Sarah E.; Nass, Caitlyn A.; Neuman, Joshua C.; Luchtel, Rebecca A.; Dobiszewski, Sarah; Miller, Joshua W.; Vailati-Riboni, Mario; Loor, Juan J.; Schalinske, Kevin L.

In: Journal of Nutrition, Vol. 149, No. 8, 01.08.2019, p. 1369-1376.

Research output: Contribution to journalArticle

Saande, CJ, Pritchard, SK, Worrall, DM, Snavely, SE, Nass, CA, Neuman, JC, Luchtel, RA, Dobiszewski, S, Miller, JW, Vailati-Riboni, M, Loor, JJ & Schalinske, KL 2019, 'Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats', Journal of Nutrition, vol. 149, no. 8, pp. 1369-1376. https://doi.org/10.1093/jn/nxz069
Saande, Cassondra J. ; Pritchard, Samantha K. ; Worrall, Deanna M. ; Snavely, Sarah E. ; Nass, Caitlyn A. ; Neuman, Joshua C. ; Luchtel, Rebecca A. ; Dobiszewski, Sarah ; Miller, Joshua W. ; Vailati-Riboni, Mario ; Loor, Juan J. ; Schalinske, Kevin L. / Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats. In: Journal of Nutrition. 2019 ; Vol. 149, No. 8. pp. 1369-1376.
@article{c934129d69074637a2f03625136f12de,
title = "Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats",
abstract = "Background: Hyperhomocysteinemia is associated with increased cardiovascular disease risk. Whole eggs contain several nutrients known to affect homocysteine regulation, including sulfur amino acids, choline, and B vitamins. Objective: The aim of this study was to determine the effect of whole eggs and egg components (i.e., egg protein and choline) with respect to 1) homocysteine balance and 2) the hepatic expression and activity of betaine-homocysteine S-methyltransferase (BHMT) and cystathionine β-synthase (CBS) in a folate-restricted (FR) rat model of hyperhomocysteinemia. Methods: Male Sprague Dawley rats (n = 48; 6 wk of age) were randomly assigned to a casein-based diet (C; n = 12), a casein-based diet supplemented with choline (C + Cho; 1.3{\%}, wt:wt; n = 12), an egg protein-based diet (EP; n = 12), or a whole egg-based diet (WE; n = 12). At week 2, half of the rats in each of the 4 dietary groups were provided an FR (0 g folic acid/kg) diet and half continued on the folate-sufficient (FS; 0.2 g folic acid/kg) diet for an additional 6 wk. All diets contained 20{\%} (wt:wt) total protein. Serum homocysteine was measured by HPLC and BHMT and CBS expression and activity were evaluated using real-time quantitative polymerase chain reaction, Western blot, and enzyme activity. A 2-factor ANOVA was used for statistical comparisons. Results: Rats fed FR-C exhibited a 53{\%} increase in circulating homocysteine concentrations compared with rats fed FS-C (P < 0.001). In contrast, serum homocysteine did not differ between rats fed FS-C and FR-EP (P = 0.078). Hepatic BHMT activity was increased by 45{\%} and 40{\%} by the EP (P < 0.001) and WE (P = 0.002) diets compared with the C diets, respectively. Conclusions: Dietary intervention with egg protein prevented elevated circulating homocysteine concentrations in a rat model of hyperhomocysteinemia, due in part to upregulation of hepatic BHMT. These data may support the inclusion of egg protein for dietary recommendations targeting hyperhomocysteinemia prevention.",
keywords = "betaine-homocysteine S-methyltransferase, cardiovascular disease, egg protein, folate-restricted diet, hyperhomocysteinemia, rat, whole egg",
author = "Saande, {Cassondra J.} and Pritchard, {Samantha K.} and Worrall, {Deanna M.} and Snavely, {Sarah E.} and Nass, {Caitlyn A.} and Neuman, {Joshua C.} and Luchtel, {Rebecca A.} and Sarah Dobiszewski and Miller, {Joshua W.} and Mario Vailati-Riboni and Loor, {Juan J.} and Schalinske, {Kevin L.}",
year = "2019",
month = "8",
day = "1",
doi = "10.1093/jn/nxz069",
language = "English (US)",
volume = "149",
pages = "1369--1376",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "8",

}

TY - JOUR

T1 - Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats

AU - Saande, Cassondra J.

AU - Pritchard, Samantha K.

AU - Worrall, Deanna M.

AU - Snavely, Sarah E.

AU - Nass, Caitlyn A.

AU - Neuman, Joshua C.

AU - Luchtel, Rebecca A.

AU - Dobiszewski, Sarah

AU - Miller, Joshua W.

AU - Vailati-Riboni, Mario

AU - Loor, Juan J.

AU - Schalinske, Kevin L.

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Background: Hyperhomocysteinemia is associated with increased cardiovascular disease risk. Whole eggs contain several nutrients known to affect homocysteine regulation, including sulfur amino acids, choline, and B vitamins. Objective: The aim of this study was to determine the effect of whole eggs and egg components (i.e., egg protein and choline) with respect to 1) homocysteine balance and 2) the hepatic expression and activity of betaine-homocysteine S-methyltransferase (BHMT) and cystathionine β-synthase (CBS) in a folate-restricted (FR) rat model of hyperhomocysteinemia. Methods: Male Sprague Dawley rats (n = 48; 6 wk of age) were randomly assigned to a casein-based diet (C; n = 12), a casein-based diet supplemented with choline (C + Cho; 1.3%, wt:wt; n = 12), an egg protein-based diet (EP; n = 12), or a whole egg-based diet (WE; n = 12). At week 2, half of the rats in each of the 4 dietary groups were provided an FR (0 g folic acid/kg) diet and half continued on the folate-sufficient (FS; 0.2 g folic acid/kg) diet for an additional 6 wk. All diets contained 20% (wt:wt) total protein. Serum homocysteine was measured by HPLC and BHMT and CBS expression and activity were evaluated using real-time quantitative polymerase chain reaction, Western blot, and enzyme activity. A 2-factor ANOVA was used for statistical comparisons. Results: Rats fed FR-C exhibited a 53% increase in circulating homocysteine concentrations compared with rats fed FS-C (P < 0.001). In contrast, serum homocysteine did not differ between rats fed FS-C and FR-EP (P = 0.078). Hepatic BHMT activity was increased by 45% and 40% by the EP (P < 0.001) and WE (P = 0.002) diets compared with the C diets, respectively. Conclusions: Dietary intervention with egg protein prevented elevated circulating homocysteine concentrations in a rat model of hyperhomocysteinemia, due in part to upregulation of hepatic BHMT. These data may support the inclusion of egg protein for dietary recommendations targeting hyperhomocysteinemia prevention.

AB - Background: Hyperhomocysteinemia is associated with increased cardiovascular disease risk. Whole eggs contain several nutrients known to affect homocysteine regulation, including sulfur amino acids, choline, and B vitamins. Objective: The aim of this study was to determine the effect of whole eggs and egg components (i.e., egg protein and choline) with respect to 1) homocysteine balance and 2) the hepatic expression and activity of betaine-homocysteine S-methyltransferase (BHMT) and cystathionine β-synthase (CBS) in a folate-restricted (FR) rat model of hyperhomocysteinemia. Methods: Male Sprague Dawley rats (n = 48; 6 wk of age) were randomly assigned to a casein-based diet (C; n = 12), a casein-based diet supplemented with choline (C + Cho; 1.3%, wt:wt; n = 12), an egg protein-based diet (EP; n = 12), or a whole egg-based diet (WE; n = 12). At week 2, half of the rats in each of the 4 dietary groups were provided an FR (0 g folic acid/kg) diet and half continued on the folate-sufficient (FS; 0.2 g folic acid/kg) diet for an additional 6 wk. All diets contained 20% (wt:wt) total protein. Serum homocysteine was measured by HPLC and BHMT and CBS expression and activity were evaluated using real-time quantitative polymerase chain reaction, Western blot, and enzyme activity. A 2-factor ANOVA was used for statistical comparisons. Results: Rats fed FR-C exhibited a 53% increase in circulating homocysteine concentrations compared with rats fed FS-C (P < 0.001). In contrast, serum homocysteine did not differ between rats fed FS-C and FR-EP (P = 0.078). Hepatic BHMT activity was increased by 45% and 40% by the EP (P < 0.001) and WE (P = 0.002) diets compared with the C diets, respectively. Conclusions: Dietary intervention with egg protein prevented elevated circulating homocysteine concentrations in a rat model of hyperhomocysteinemia, due in part to upregulation of hepatic BHMT. These data may support the inclusion of egg protein for dietary recommendations targeting hyperhomocysteinemia prevention.

KW - betaine-homocysteine S-methyltransferase

KW - cardiovascular disease

KW - egg protein

KW - folate-restricted diet

KW - hyperhomocysteinemia

KW - rat

KW - whole egg

UR - http://www.scopus.com/inward/record.url?scp=85071055313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071055313&partnerID=8YFLogxK

U2 - 10.1093/jn/nxz069

DO - 10.1093/jn/nxz069

M3 - Article

C2 - 31111947

AN - SCOPUS:85071055313

VL - 149

SP - 1369

EP - 1376

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 8

ER -